Anti-PSMA/FOLH1 Monoclonal Antibody for WB, IHC-P, ELISA - Q04609

Core Product Specifications and Parameters

Biological Background: PSMA/FOLH1 Function and Localization

• PSMA/FOLH1 (Glutamate carboxypeptidase 2) is a multifunctional enzyme that possesses both folate hydrolase and N-acetylated-alpha-linked acidic dipeptidase (NAALADase) activity, with preference for tri-alpha-glutamate peptides.
• In the intestine, it facilitates folate absorption by cleaving polyglutamated folates, while in the brain, it modulates glutamatergic neurotransmission by hydrolyzing the neuropeptide NAAG to release glutamate.
• The protein is involved in prostate tumor progression, and isoform-specific expression has been observed: PSMA' predominates in normal prostate, PSMA-1 in primary tumors, and PSMA-9 is specifically expressed in prostate cancer.
• Subcellularly, it is anchored to the cell membrane via a signal-anchor transmembrane domain, but also localizes to the cytoplasm.
• It additionally exhibits dipeptidyl-peptidase IV-like activity, cleaving substrates such as Gly-Pro-AMC in vitro.
• As a zinc-dependent metalloprotease, it requires zinc and calcium for catalytic function and undergoes glycosylation and phosphorylation, contributing to an apparent molecular weight higher than the predicted 84 kDa.
• Tissue distribution is broad but highest in prostate epithelium, with significant expression also in kidney, brain, small intestine, and various tumor endothelia.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the extracellular domain (aa 50-150) of human PSMA/FOLH1, which is accessible in native and denaturing conditions, supporting its use in WB, IHC-P, and ELISA applications.
• In western blotting, note that glycosylation may cause the band to migrate at 100–120 kDa rather than the predicted 84 kDa; validate the observed band using positive controls or knockdown/knockout samples.
• For IHC-P, antigen retrieval with citrate or EDTA buffer (pH 6.0–9.0) is often beneficial; titration and optimization in the relevant tissue type are recommended.
• Cross-reactivity with mouse and rat allows preclinical studies in common models; confirm endogenous expression levels in the tissue/cell line of interest before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, bridging the gap from concept to clinic with a comprehensive portfolio tailored to diagnostic developers. For research areas centered on PSMA/FOLH1—such as prostate cancer diagnostics, neurobiology, and folate metabolism—we supply validated antibody pairs, optimized rabbit monoclonal antibodies like this anti-PSMA/FOLH1 clone, and auxiliary reagents to support your assay development. Whether you need bulk raw materials or rare-target reagents, our one-stop service model streamlines procurement so you can focus on translating discoveries into reliable diagnostic tests.