Anti-PSMA7 Rabbit Monoclonal Antibody (KD Validated) for WB, IHC-P, IF/ICC, ELISA - O14818

Core Product Specifications and Parameters

Biological Background: PSMA7 Function and Localization

• Component of the 20S core proteasome complex; involved in proteolytic degradation of most intracellular proteins.
• Forms the 26S proteasome with two 19S regulatory particles, mediating ATP-dependent degradation of ubiquitinated proteins.
• Associated with PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.
• Inhibits HIF-1A transactivation function; promotes HIF-1A degradation under hypoxia via EMAP2 interaction.
• Plays a role in hepatitis C virus internal ribosome entry site-mediated translation.
• Mediates nuclear translocation of androgen receptor (AR), enhancing androgen-mediated transactivation.
• Promotes MAVS degradation, negatively regulating MAVS-mediated innate immune response.
• Subcellularly located in both cytoplasm and nucleus. Post-translational modifications include acetylation, phosphorylation, and glycosylation; alternative splicing isoforms exist.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 150–248 of human PSMA7; ensure the epitope is accessible in your specific assay format.
• For Western blot (WB), consider using whole-cell lysates from human or mouse tissues/cells; the predicted molecular weight is 28 kDa.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; validate with appropriate positive and negative controls.
• For immunofluorescence (IF/ICC), test cell fixation and permeabilization conditions to optimize signal.
• For ELISA, confirm the antibody works in your sandwich or indirect ELISA setup with recombinant PSMA7 or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies like this anti-PSMA7 clone, and bulk ancillary reagents for diagnostic manufacturers and research laboratories. We also assist in sourcing rare target raw materials to support your proteasome research, protein degradation, and cancer biomarker studies. For tailored procurement solutions, contact our team.