Anti-PSMA6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P60900

Core Product Specifications and Parameters

Biological Background: PSMA6 Function and Localization

• PSMA6, also known as proteasome subunit alpha type-6, PROS-27, or p27K, is a core component of the 20S proteasome complex, a large proteinase responsible for degrading intracellular proteins.
• As part of the 20S core particle, it contributes to the assembly of the 26S proteasome when associated with 19S regulatory particles, mediating ATP-dependent degradation of ubiquitinated proteins.
• PSMA6 is also involved in ubiquitin-independent proteolysis through association with PA200 or PA28 regulatory complexes, playing roles in spermatogenesis and antigenic peptide generation for MHC class I.
• The protein localizes to both the cytoplasm and nucleus, reflecting the dynamic distribution of proteasomes in cells.
• Post-translational modifications include acetylation, phosphorylation, and glycosylation, as identified by mass spectrometry and direct protein sequencing.
• PSMA6 is implicated in maintaining protein homeostasis by removing misfolded or damaged proteins and regulating cellular processes through timely degradation of regulatory proteins.
• Alternative splicing has been reported for the PSMA6 gene, potentially generating multiple isoforms.

Experimental Guidance and Technical Tips

• For western blot (WB), consider using whole cell lysates from human, mouse, or rat samples, as cross reactivity has been indicated for these species.
• In immunofluorescence (IF/ICC), fixation and permeabilization conditions should be optimized, and appropriate secondary antibody detection should be validated.
• For ELISA applications, coating with recombinant or purified PSMA6 protein may serve as a positive control to assess antibody binding.
• The immunogen corresponds to the full-length human PSMA6 (amino acids 1–246), raising the possibility that the antibody may recognize native protein under non-denaturing conditions; appropriate controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, researchers, and labs with a streamlined channel for sourcing IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our portfolio supports proteasome research and related protein degradation pathways, ensuring rapid access to rare-target antibodies and production-scale raw materials. From concept to clinic, CamelBio streamlines your procurement with one-stop convenience.