Anti-PSMA6 Polyclonal Antibody for WB, IF/ICC, ELISA - P60900

Core Product Specifications and Parameters

Biological Background: PSMA6 Function and Localization

• PSMA6 encodes the proteasome subunit alpha type-6, also designated as the iota chain, p27K, PROS27, and macropain iota chain.
• As a core component of the 20S proteasome, PSMA6 is integral to the proteolytic degradation of the majority of intracellular proteins.
• Incorporating into the 26S proteasome via association with two 19S regulatory particles, it contributes to ATP- and ubiquitin-dependent protein turn-over, essential for maintaining protein homeostasis and removing aberrant proteins.
• The 20S proteasome can also bind PA200 or PA28 activators to enable ubiquitin-independent degradation, processes critical in spermatogenesis and MHC class I antigenic peptide generation, respectively.
• PSMA6 localises to both the cytoplasm and the nucleus, reflecting the broad intracellular distribution of proteasomes.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and glycosylation, and exists in alternatively spliced isoforms, as identified by direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 1-246; this region may be conserved across species, consistent with the reported human, mouse, and rat cross-reactivity.
• For Western blotting, a specific band near 27 kDa is expected; verify performance in suitable positive control lysates (e.g., HeLa, HEK293, mouse or rat tissues).
• In immunofluorescence or ICC, consider appropriate fixation and permeabilization protocols, as proteasome subunits may be present in both nuclear and cytoplasmic compartments.
• ELISA applications can be optimized by using the recombinant immunogen as a coating antigen to confirm antibody reactivity.
• As a polyclonal antibody, lot-to-lot consistency should be assessed; including a loading control and pre-immune serum control is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to essential IVD raw materials, including validated polyclonal antibodies like this anti-PSMA6 product for studying proteasome biology and protein degradation pathways. Our portfolio extends to antibody pairs, recombinant antigens, and ancillary reagents, supporting workflows from biomarker discovery to assay development. We excel at sourcing rare targets and delivering bulk quantities, helping you accelerate every stage—from concept to clinic.