Anti-PSMA5 Rabbit Polyclonal Antibody for WB, IP, ELISA - P28066

Core Product Specifications and Parameters

Biological Background: PSMA5 Function and Localization

• PSMA5, also known as Proteasome subunit alpha type-5 or macropain zeta chain, is a core alpha subunit of the 20S proteasome complex.
• It is essential for the proteolytic degradation of most intracellular proteins, helping to remove misfolded, damaged, or no-longer-needed proteins.
• Together with 19S regulatory particles, PSMA5 forms the 26S proteasome, which performs ATP-dependent degradation of ubiquitinated proteins to maintain protein homeostasis.
• PSMA5 also participates in ubiquitin-independent degradation via association with PA200 or PA28 regulatory complexes, involved in processes such as spermatogenesis (PA200) and MHC class I antigen presentation (PA28).
• The protein localizes to both the cytoplasm and nucleus.
• Post-translational modifications include acetylation, phosphorylation, and glycosylation, as suggested by proteomic analyses.
• Expression has been detected at the protein level in fetal brain tissue.

Experimental Guidance and Technical Tips

• For western blotting, the predicted protein weight of 26 kDa suggests using 10–15% SDS-PAGE gels for optimal resolution. Validate detection in relevant sample systems.
• The rabbit polyclonal antibody may recognize PSMA5 in human, mouse, and rat lysates; however, cross-reactivity should be verified in the target species if not previously confirmed.
• In immunoprecipitation assays, the full-length recombinant protein immunogen likely enables efficient capture; consider including positive and negative controls to assess IP performance.
• For ELISA applications, both direct and sandwich formats can be explored, but optimal working concentrations should be determined empirically with appropriate antigen standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, including high-quality antibodies like this anti-PSMA5 polyclonal antibody for proteasome research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development pipelines. For niche targets such as PSMA5, our team can assist with customized sourcing and technical consultation to help you move from concept to clinic efficiently.