Anti-PSMA5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P28066

Core Product Specifications and Parameters

Biological Background: PSMA5 Function and Localization

• PSMA5 (proteasome subunit alpha type-5), also referred to as macropain zeta chain, multicatalytic endopeptidase complex zeta chain, or proteasome zeta chain, is a core subunit of the 20S proteasome complex.
• As part of the 20S core proteasome, PSMA5 is involved in the proteolytic degradation of most intracellular proteins. When associated with two 19S regulatory particles to form the 26S proteasome, it facilitates ATP-dependent degradation of ubiquitinated proteins, crucial for protein homeostasis.
• In its PA200- or PA28-associated form, PSMA5 participates in ubiquitin-independent protein degradation, which is important for processes such as spermatogenesis and the generation of MHC class I-presented antigenic peptides.
• The PSMA5 gene is a member of the proteasome alpha subunit family and encodes a protein that localizes to both the cytoplasm and nucleus.
• PSMA5 is subject to several post-translational modifications, including acetylation, phosphorylation, and glycosylation, which may regulate its function.
• At the protein level, PSMA5 expression has been detected in fetal brain.
• Proteasome subunit alpha type-5 has been identified through direct protein sequencing and large-scale proteomics, and the P28066 entry is part of the UniProtKB/Swiss-Prot reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human PSMA5 protein (amino acids 1-241). This design may provide broad reactivity across isoforms and species due to conserved sequences.
• For Western blot, the predicted molecular weight is approximately 26kDa; however, post-translational modifications may cause the protein to migrate slightly differently. Validate the observed band in your sample system.
• This antibody is suggested for use in WB, IHC-P, IF/ICC, and ELISA. For IHC-P and IF/ICC, appropriate antigen retrieval and permeabilization steps should be optimized for your tissue or cell type.
• Demonstrated cross-reactivity with mouse and rat indicates potential utility in multi-species studies. It is advisable to verify specificity in the target species of interest.
• In ELISA applications, consider the detection system and plate coating conditions to maximize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For your proteasome research needs, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to accelerate your project from concept to clinic.