Anti-PSMA4 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P25789

Core Product Specifications and Parameters

Biological Background: PSMA4 Function and Localization

• PSMA4 encodes the proteasome subunit alpha type-4, a core component of the 20S proteasome, also designated as subunit C9, proteasome subunit L, or alpha-3.
• As part of the 20S core particle, PSMA4 contributes to the barrel-shaped structure that proteolytically degrades most intracellular proteins in an ATP/ubiquitin-dependent manner via the 26S proteasome (20S + 19S regulatory particles) and also mediates ubiquitin-independent degradation through association with PA200 or PA28 regulators.
• The ubiquitin-independent degradation pathways involving PSMA4 are critical for spermatogenesis (20S-PA200 complex) and the generation of MHC class I-presented antigenic peptides (20S-PA28 complex), highlighting its role in both reproductive biology and adaptive immunity.
• PSMA4 localizes predominantly to the cytoplasm and nucleus, consistent with the broad intracellular distribution of the proteasome system for protein quality control and regulated proteolysis.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may modulate proteasome activity or assembly, and is subject to alternative splicing, potentially generating isoforms with distinct functions.
• The presence of a host-virus interaction keyword suggests that PSMA4 and the proteasome may be exploited by viral pathogens, an area of active investigation in infection biology.
• The proteasome alpha subunits, including PSMA4, form the outer rings of the 20S core and gate substrate entry, with specific roles in recognizing and unfolding substrates prior to degradation.

Experimental Guidance and Technical Tips

• The antibody was generated against full-length human PSMA4 recombinant protein, which shares high sequence homology with mouse and rat orthologs; thus, it is expected to recognize PSMA4 from these species, but verification in the specific sample type is recommended.
• For Western blotting, the predicted molecular weight is ~29 kDa; however, due to potential post-translational modifications, actual band migration may vary; ensure appropriate gel percentage and transfer conditions.
• In immunoprecipitation, the polyclonal nature may capture the native PSMA4 and potentially associated proteasome complexes; consider cross-linking protocols if preserving protein interactions.
• For immunofluorescence/ICC, PSMA4 localizes to both cytoplasm and nucleus; permeabilization and fixation methods may affect staining patterns; include appropriate controls.
• ELISA applications may benefit from using a matched antibody pair for sandwich assays; validate with recombinant PSMA4 standard curves.
• Since PSMA4 is an abundant housekeeping protein, loading controls like beta-actin or GAPDH may not reflect accurate normalization; consider total protein staining or proteasome-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibodies for proteasome targets like PSMA4. Our portfolio includes optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support proteasome-related assay development and biomarker research. Partner with CamelBio for reliable, end-to-end procurement from concept to clinic.