Anti-PSMA4 Rabbit Polyclonal Antibody for WB, ELISA - P25789

Core Product Specifications and Parameters

Biological Background: PSMA4 Function and Localization

• PSMA4 (Proteasome subunit alpha type-4), also known as Macropain subunit C9, HC9, PSC9, is a core component of the 20S proteasome complex.
• As part of the 20S core, it associates with 19S regulatory particles to form the 26S proteasome, which mediates ATP-dependent degradation of ubiquitinated proteins, crucial for protein homeostasis and removal of misfolded or damaged proteins.
• The 20S proteasome can also partner with PA200 or PA28 regulators for ubiquitin-independent protein degradation, playing key roles in spermatogenesis (20S-PA200) and generation of MHC class I antigenic peptides (20S-PA28).
• PSMA4 localizes to both the cytoplasm and nucleus, indicating its involvement in proteasomal degradation across cellular compartments.
• Post-translational modifications identified by mass spectrometry include acetylation and phosphorylation, which may regulate proteasome activity or assembly.
• The gene undergoes alternative splicing and the protein is implicated in host-virus interactions, highlighting its diverse cellular roles.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the full-length human PSMA4 (amino acids 1-261). This broad coverage may enable detection of both native and denatured forms of the protein in WB and ELISA.
• For Western blotting, consider optimizing antibody dilution and blocking conditions using lysates from human, mouse, rat, or monkey cells, given the confirmed cross-reactivity with these species.
• For ELISA applications, validate the assay format by confirming coating antigen suitability and detection range in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering one-stop access to validated antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your diagnostic development. For proteasome-related research, we provide high-quality anti-PSMA4 antibodies alongside optimized monoclonal/polyclonal options and technical consulting to support your assay workflows from concept to clinic.