Anti-PSMA3 Rabbit Polyclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - P25788

Core Product Specifications and Parameters

Biological Background: PSMA3 (Proteasome subunit alpha type-3) Function and Localization

• PSMA3, also known as Proteasome subunit alpha type-3, Macropain subunit C8, or HC8, is a core component of the 20S proteasome complex, which mediates the proteolytic degradation of most intracellular proteins.
• As part of the 26S proteasome (20S core plus two 19S regulatory particles), PSMA3 participates in ATP-dependent degradation of ubiquitinated proteins, crucial for maintaining protein homeostasis by removing misfolded or damaged proteins.
• In association with alternative regulatory particles like PA200 or PA28, the 20S proteasome carries out ubiquitin-independent protein degradation, involved in processes such as spermatogenesis (with PA200) and generation of MHC class I antigenic peptides (with PA28).
• PSMA3 localizes to both the cytoplasm and the nucleus, consistent with the broad distribution of proteasomes for protein quality control and regulated proteolysis.
• This subunit binds the C-terminus of CDKN1A (p21), mediating its degradation, and negatively regulates membrane trafficking of the thromboxane A2 receptor (TBXA2R) isoform 2.
• PSMA3 is subject to post-translational modifications including acetylation and phosphorylation, and is classified under several keywords: 3D-structure, Alternative splicing, Host-virus interaction, Phosphoprotein, Proteasome, and Proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody consists of a recombinant protein covering amino acids 1–255 of human PSMA3, encompassing most of the full-length protein; this design may provide robust recognition of the native target.
• Given its predicted molecular weight of approximately 28 kDa, ensure appropriate gel percentage (e.g., 10–12% SDS-PAGE) and transfer conditions for Western blot detection.
• The antibody has been reported to work in WB, IP, IF-P, IHC-P, and ELISA across human, mouse, and rat samples; for other species or applications, validation in the relevant sample system is recommended.
• For immunoprecipitation or immunofluorescence, consider the abundant cytoplasmic/nuclear distribution of the target; appropriate lysis buffers and fixation/permeabilization protocols should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing validated IVD raw materials and one-stop sourcing solutions for diagnostic manufacturers and research laboratories. This anti-PSMA3 polyclonal antibody, targeting a key proteasome subunit involved in protein degradation and cellular homeostasis, exemplifies our commitment to supplying high-quality reagents for proteostasis and ubiquitin-proteasome pathway studies. Beyond individual antibodies, we offer bulk quantities, matched antibody pairs, and tailored procurement for rare targets to streamline your development pipeline from concept to clinic.