Anti-PSMA3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P25788

Core Product Specifications and Parameters

Biological Background: PSMA3 Function and Localization

• PSMA3, also known as proteasome subunit alpha type-3, macropain subunit C8, or proteasome subunit alpha-7, is a core component of the 20S proteasome complex.
• It participates in the ATP-dependent degradation of ubiquitinated proteins as part of the 26S proteasome, playing a critical role in maintaining protein homeostasis by removing misfolded or damaged proteins.
• When associated with PA200 or PA28 regulatory particles, the 20S proteasome mediates ubiquitin-independent degradation, essential for spermatogenesis and generation of certain MHC class I antigenic peptides.
• PSMA3 is localized in both the cytoplasm and nucleus, aligning with its proteolytic functions throughout the cell.
• It directly interacts with CDKN1A, facilitating its degradation, and negatively regulates membrane trafficking of the thromboxane A2 receptor isoform 2.
• Post-translational modifications include acetylation and phosphorylation, and the protein is implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein immunogen, which may represent full-length or a fragment of PSMA3; this design typically yields high specificity and strong affinity.
• For Western blotting, the expected band size is approximately 28kDa; consider using well-characterized positive controls (e.g., HeLa or Jurkat lysates) and validate reactivity in your sample system.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., citrate buffer, pH 6.0) may be required; optimize antibody dilution and detection conditions for your tissue type.
• For ELISA applications, the antibody can serve as a capture or detection reagent; pairing with another anti-PSMA3 clone may be explored for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PSMA3 monoclonal antibody is part of our extensive portfolio of proteasome-targeting reagents, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary proteins. Our supply capabilities encompass rare target raw-material sourcing and custom conjugation, supporting your assay development with reliable B2B efficiency.