Anti-PSMA2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P25787

Core Product Specifications and Parameters

Biological Background: PSMA2 Function and Localization

• PSMA2 (Proteasome subunit alpha type-2) is a core component of the 20S proteasome complex, which is responsible for the proteolytic degradation of most intracellular proteins.
• It plays an essential role in maintaining protein homeostasis by associating with different regulatory particles; the 26S proteasome (20S core plus two 19S regulatory particles) mediates ATP-dependent degradation of ubiquitinated proteins, clearing misfolded, damaged, or unnecessary proteins.
• The 20S proteasome can also mediate ubiquitin-independent protein degradation when associated with PA200 or PA28, contributing to pathways such as spermatogenesis and generation of MHC class I-presented antigenic peptides.
• PSMA2 localizes to both the cytoplasm and nucleus, consistent with the proteasome's broad cellular distribution.
• Post-translational modifications include acetylation and phosphorylation; keywords indicate it is a phosphoprotein with direct protein sequencing evidence.
• The molecular weight is approximately 26 kDa (UniProt: 25899 Da), matching the observed 26kDa band in western blot analysis.
• PSMA2 belongs to the peptidase T1A family and is also known by aliases such as MU, HC3, PSC2, and PMSA2.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–234) of human PSMA2, which may confer high specificity for the target. Consider validating the antibody in relevant cell lines or tissue lysates to confirm reactivity.
• For western blot (WB), whole cell lysates from human, mouse, or rat samples can be used; expect a band around 26 kDa. Including a positive control (e.g., HeLa, HEK293) is advisable.
• For immunofluorescence (IF/ICC), PSMA2 is expected to exhibit both nuclear and cytoplasmic staining. Permeabilization conditions may need optimization.
• ELISA applications can be developed using recombinant PSMA2 protein to establish a standard curve. Pairing with appropriate capture/detection antibodies may require empirical testing.
• Cross-reactivity with mouse and rat allows for comparative studies, but sequence homology should be verified if using knockout or knockdown models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting projects from concept to clinic. For proteasome research and PSMA2-targeted assays, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our team also assists in rare-target raw-material sourcing to accelerate your immunoassay development and biomarker discovery programs.