Anti-PSMA2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P25787

Core Product Specifications and Parameters

Biological Background: PSMA2 Function and Localization

• PSMA2 (proteasome subunit alpha type-2), also known as macropain subunit C3, multicatalytic endopeptidase complex subunit C3, and proteasome component C3, is a core component of the 20S proteasome complex.
• As part of the 20S core, PSMA2 plays a critical role in the proteolytic degradation of most intracellular proteins, maintaining protein homeostasis by removing misfolded or damaged proteins and degrading proteins whose functions are no longer required.
• PSMA2 associates with different regulatory particles: with two 19S regulatory particles it forms the 26S proteasome, which mediates ATP-dependent degradation of ubiquitinated proteins; with PA200 or PA28 it forms complexes that perform ubiquitin-independent protein degradation in processes such as spermatogenesis and MHC class I antigen presentation.
• The PSMA2 gene encodes a 234-amino acid protein with a calculated molecular weight of approximately 25.9 kDa, and it undergoes post-translational modifications including acetylation and phosphorylation.
• PSMA2 localizes to both the cytoplasm and the nucleus, consistent with the broad distribution of the proteasome system.
• PSMA2 is part of a large proteasome gene family (alpha-type subunits), and its expression is conserved across human, mouse, and rat, enabling cross-species detection.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human PSMA2. This region is highly conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting (WB), a specific band at approximately 26 kDa is expected for endogenous PSMA2. Consider using a reference cell line lysate (e.g., HeLa or HepG2) to confirm specificity.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), both cytoplasmic and nuclear staining may be observed, reflecting the subcellular localization of the proteasome complex. Validate in the relevant tissue or cell system, and include appropriate controls (e.g., peptide blocking) to ensure signal specificity.
• For ELISA applications, the antibody may be used as a capture or detection reagent; optimization of coating concentration and detection conditions is recommended.
• This antibody has not been tested in other applications; if planning to use in immunoprecipitation (IP) or flow cytometry, validate performance under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for sourcing high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers studying the ubiquitin-proteasome system or protein degradation pathways, CamelBio can help streamline your supply chain from discovery to production. Our sourcing capabilities cover a broad range of molecular biology applications, ensuring reliable access to the tools you need.