Anti-PSMA1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P25786

Core Product Specifications and Parameters

Biological Background: PSMA1 Function and Localization

• Protein identity: PSMA1, also known as proteasome subunit alpha type-1, PROS-30, HC2, NU, and HEL-S-275, is a core component of the 20S proteasome complex.
• Core function: As part of the 20S core, PSMA1 contributes to the proteolytic degradation of most intracellular proteins. The complex can associate with regulatory particles to form the 26S proteasome for ATP-dependent degradation of ubiquitinated proteins, or with PA200 or PA28 for ubiquitin-independent protein degradation.
• Biological roles: The 26S proteasome maintains protein homeostasis by clearing misfolded or damaged proteins and regulatory proteins no longer needed. The 20S-PA200 complex is required in spermatogenesis, while the 20S-PA28 complex generates a subset of MHC class I-presented antigenic peptides, implicating PSMA1 in immune function.
• Gene family context: Encoded by the PSMA1 gene (GeneID: 5682) in humans, it belongs to the alpha-type proteasome subunit family. Alternative splicing has been noted.
• Subcellular localization: PSMA1 is found in both the cytoplasm and nucleus, consistent with its role in protein degradation throughout the cell.
• Post-translational modifications and additional features: According to UniProt, PSMA1 undergoes acetylation, phosphorylation, glycosylation, and Ubl conjugation. It also contains isopeptide bonds. These modifications may regulate its activity or assembly.
• Tissue and functional specificity: While widely expressed, its involvement in spermatogenesis and antigen processing indicates specialized functions in reproductive and immune tissues.

Experimental Guidance and Technical Tips

• Immunogen design: This rabbit monoclonal antibody was raised against a synthetic peptide spanning amino acids 164–263 of human PSMA1. This region likely offers specificity for the target.
• Western blot (WB): Based on the predicted molecular weight (30 kDa), a standard SDS-PAGE protocol is expected to detect PSMA1 in human, mouse, and rat samples. Consider using appropriate positive controls and optimizing primary antibody dilution.
• Immunofluorescence/immunocytochemistry (IF/ICC): Since PSMA1 localizes to both cytoplasm and nucleus, fixation and permeabilization conditions should preserve both compartments. Validate signal specificity with known localization patterns.
• ELISA: This antibody can be used in ELISA applications; consider its utility in capture or detection formats after in-house validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers, laboratories, and research institutes, offering comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For proteasome-related research and assay development, CamelBio can provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing solutions for rare targets. We assist in streamlining your procurement, ensuring you have the right tools to advance your projects efficiently.