Anti-PSIP1 Rabbit Polyclonal Antibody for WB, ELISA - O75475

Core Product Specifications and Parameters

Biological Background: PSIP1 Function and Localization

• PSIP1, also known as LEDGF, DFS70, p75/p52, and PAIP, is a transcriptional coactivator that plays a critical role in neuroepithelial stem cell differentiation and neurogenesis, particularly in lens epithelial cell gene regulation and stress responses.
• The protein exists as multiple isoforms; isoform 2 (p75) acts as a more general and stronger transcriptional coactivator and may also function as an adapter to coordinate pre-mRNA splicing, while isoform 1 (p52) is less characterized.
• PSIP1 acts as a cellular cofactor for lentiviral integration, tethering pre-integration complexes to host chromatin via its integrase-binding domain, a function critical for HIV-1 replication.
• Subcellular localization is exclusively nuclear, consistent with its roles in transcription, splicing, and viral integration.
• The protein is widely expressed, with highest levels in the thymus and pronounced expression in fetal and adult brain, including the subventricular zone, hippocampus, and germinal neuroepithelium; expression is markedly higher in fetal brain than adult.
• Post-translational modifications include phosphorylation, citrullination, and ubiquitin conjugation, which may regulate its activity and interactions.
• Additional structural features include a coiled-coil domain and a DNA-binding region, facilitating its chromatin association and transcriptional regulation.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant protein immunogen spanning amino acids 301-530 of human PSIP1, which corresponds to the C-terminal region encompassing the integrase-binding domain and part of the coiled-coil domain; it is expected to recognize both major isoforms (p75 and p52).
• For Western blot (WB), consider using nuclear extracts from relevant cell lines such as HeLa or neuronal precursors, and note the predicted band around 60 kDa; due to isoform variation and post-translational modifications, multiple bands may be observed.
• Cross-reactivity with mouse and rat makes this antibody suitable for comparative studies in rodent models, but validate in the relevant sample system to confirm specificity.
• For ELISA, consider using recombinant protein or peptide antigens to assess binding affinity; direct ELISA may be used for titer determination, while sandwich ELISA may require paired antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop sourcing partner for IVD manufacturers, research laboratories, and diagnostic developers, offering seamless access to validated raw materials, technical services, and consulting from concept to clinic. For your research involving PSIP1 and its roles in transcription, lens differentiation, neurogenesis, or lentiviral integration, we can provide optimized monoclonal and polyclonal antibodies, matched antigen pairs, and bulk ancillary reagents. Our global network ensures timely delivery of rare target raw materials and supports your assay development with expert guidance, helping you accelerate projects with confidence.