Anti-PSG1 Polyclonal Antibody for WB/IF-P/IHC-P/ELISA - P11464

Core Product Specifications and Parameters

Biological Background: PSG1 Function and Localization

• PSG1 (Pregnancy-specific beta-1-glycoprotein 1) belongs to the carcinoembryonic antigen (CEA) family and contains immunoglobulin (Ig)-like domains.
• It is a secreted glycoprotein, consistent with the presence of an N-terminal signal peptide and extracellular localization.
• The protein undergoes extensive post-translational modifications, including N-linked glycosylation and formation of intramolecular disulfide bonds.
• Alternative splicing generates multiple PSG1 isoforms, which may vary in domain composition and biological activity.
• Sequence analysis reveals repeated Ig-like domains, a characteristic feature of the CEA family members.
• Keywords associated with the UniProt entry include Alternative splicing, Disulfide bond, Glycoprotein, Immunoglobulin domain, Proteomics identification, Reference proteome, Repeat, Secreted, and Signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 180–419 of human PSG1, covering the C-terminal region rich in Ig-like domains. This design may enable recognition of multiple isoforms sharing this sequence.
• For Western blot, the predicted molecular weight is approximately 47 kDa; however, due to glycosylation, the observed band may migrate at a higher apparent molecular weight. Use appropriate positive controls, such as placental tissue lysates or PSG1-overexpressing cell lines.
• Antibody reactivity in immunofluorescence (IF-P) and immunohistochemistry (IHC-P) should be validated in relevant sample systems where PSG1 is known to be expressed (e.g., placenta or trophoblast cell lines).
• In ELISA, confirm binding specificity using recombinant PSG1 protein and optimize dilution factors for the detection system.
• Note that this is a polyclonal antibody; lot-to-lot variability may occur, and titration is recommended for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering seamless access to diagnostic antibodies, antigen pairs, and ancillary reagents. This anti-PSG1 polyclonal antibody exemplifies our capability to supply high-quality immunoreagents targeting pregnancy-related biomarkers. Beyond single products, we support manufacturers with bulk procurement, custom antibody development, and sourcing of rare targets—helping streamline your concept-to-clinic pipeline.