Anti-PSEN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P49810

Core Product Specifications and Parameters

Biological Background: Presenilin-2 Function and Localization

• Presenilin-2 (PSEN2) is the catalytic subunit of the gamma-secretase complex, which mediates intramembrane proteolysis of substrates including amyloid-beta precursor protein (APP) and Notch receptors, producing amyloid beta peptides (PubMed:10497236, PubMed:10652302, PubMed:16752394). Related references: PMID:10497236 PMID:10652302 PMID:16752394

• PSEN2 preferentially cleaves substrates in late endosomal/lysosomal compartments, generating the intracellular pool of longer amyloid beta species (PubMed:27293189). Related references: PMID:27293189

• PSEN2 acts as a calcium-leak channel at the endoplasmic reticulum, passively transporting calcium to the cytosol, and is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and calcium shuttling (PubMed:16959576, PubMed:21285369). Related references: PMID:16959576 PMID:21285369

• The protein localizes to the endoplasmic reticulum, Golgi apparatus, late endosome, and lysosome membranes.

• PSEN2 is expressed in multiple tissues including placenta, skeletal muscle, heart, brain, liver, and kidney, and shows somatodendritic enrichment in hippocampal neurons (PubMed:8574969, PubMed:27293189). Related references: PMID:8574969 PMID:27293189

• Genetic variants of PSEN2 are implicated in Alzheimer disease and cardiomyopathy, highlighting its role in both proteolytic and calcium signaling pathways.

• Post-translational modifications such as phosphorylation regulate PSEN2 activity.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates from human, mouse, or rat cells, with an expected protein weight of approximately 50 kDa; optimize loading and detection conditions for your sample type.
• For immunofluorescence (IF/ICC), validate fixation and permeabilization protocols due to the multi-pass membrane topology of PSEN2, which may affect antibody accessibility.
• The immunogen corresponds to the C-terminal region (aa 267-384); consider pre-adsorption or blocking strategies to reduce nonspecific binding in sensitive assays.
• As a polyclonal antibody, lot-to-lot consistency should be verified when transitioning between production batches in quantitative or diagnostic applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material procurement, providing antibodies, recombinant proteins, and ancillary reagents to research and diagnostic communities. For gamma-secretase and Alzheimer's disease research, our portfolio includes validated PSEN2 antibodies, antibody pairs, and bulk raw materials tailored to assay development needs. Streamline your supply chain from discovery to clinical application with CamelBio's one-stop sourcing expertise.