Anti-PSD95 Rabbit PolymAb® for WB, IHC-P, ELISA - P78352

Core Product Specifications and Parameters

Biological Background: PSD95 Function and Localization

• Postsynaptic scaffolding protein (PSD-95) encoded by DLG4, also known as synapse-associated protein 90 (SAP-90), plays a pivotal role in organizing synaptic protein complexes.
• Critical for synaptogenesis and synaptic plasticity by clustering NMDA receptor subunits, shaker-type potassium channels, and other signaling molecules at the postsynaptic density.
• Regulates AMPA-type glutamate receptor (AMPAR) immobilization, maintaining channels in an activated state in the presence of glutamate and preventing synaptic depression (By similarity).
• Overexpression or depletion of DLG4 alters the ratio of excitatory to inhibitory synapses in hippocampal neurons, underscoring its role in balancing synaptic transmission.
• Cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation, inhibiting ZDHHC5 association with endocytic proteins. Related references: PMID:26334723
• Displays multi-compartment localization: enriched at the postsynaptic density, but also detected at cell membrane, cytoplasm, axon, dendritic spines, dendrites, and presynapses.
• Brain-specific expression; annotated with keywords including alternative splicing, disease variant (intellectual disability), lipoprotein, palmitate, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (aa 1–100) of human PSD95, which is conserved across species; verify reactivity in mouse and rat samples if cross-reactivity is expected.
• For western blotting, the predicted band is ~80 kDa; use brain tissue lysates or synaptic fractionations as a positive control.
• In IHC-P, expect punctate synaptic staining patterns; optimize antigen retrieval and antibody dilution for your tissue type.
• Consider testing dilutions starting at 1:1000 for WB and 1:100 for IHC-P; validate performance with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials. This anti-PSD95 antibody exemplifies our commitment to high-quality neuroscience reagents, and we further offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your project from concept to clinic.