Anti-PSD95 Rabbit Polyclonal Antibody for WB, ELISA - P78352

Core Product Specifications and Parameters

Biological Background: PSD95 Function and Localization

• PSD95, also known as Disks large homolog 4, SAP90, or SAP-90, is a key postsynaptic scaffolding protein encoded by the DLG4 gene. It is essential for synaptogenesis and synaptic plasticity in the mammalian brain.
• As a core component of the postsynaptic density, PSD95 facilitates the clustering of postsynaptic proteins by interacting with the cytoplasmic tails of NMDA receptor subunits, shaker-type potassium channels, and other signaling molecules, thereby organizing receptor complexes.
• PSD95 regulates the balance between excitatory and inhibitory synapses; overexpression or depletion alters the ratio in hippocampal neurons, impacting neural network formation.
• It contributes to the modulation of synaptic strength by controlling AMPA-type glutamate receptor (AMPAR) immobilization, keeping channels in an activated state and preventing synaptic depression.
• PSD95 may also regulate intracellular trafficking; it reduces the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly and may regulate the trafficking of ADR1B.
• A specific molecular mechanism involves cooperation with the tyrosine kinase FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane, a process dependent on FYN-mediated phosphorylation of ZDHHC5 and inhibition of its endocytic association. Related references: PMID:26334723
• Subcellularly, PSD95 localizes extensively to synaptic sites including the postsynaptic density, presynapse, and both axonal and dendritic projections, reflecting its role in synaptic architecture.
• Post-translational modifications such as palmitoylation and phosphorylation (evidenced by keywords) are critical for its membrane targeting and function, with additional involvement in ubiquitination and alternative splicing contributing to its regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human PSD95, which may yield antibodies recognizing full-length and N-terminal fragments; consider verifying specificity in relevant tissue lysates.
• This polyclonal antibody is suitable for Western Blot and ELISA; for WB, a band at approximately 80 kDa is expected in brain tissue samples from human, mouse, or rat.
• Due to high sequence conservation, additional cross-reactivity with other species may be possible; validation is recommended for non-listed species.
• For ELISA, the peptide coating concentration and antibody dilution should be optimized empirically.
• To confirm specificity, consider using peptide blocking experiments or knockdown/knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to validated IVD raw materials, including this polyclonal antibody targeting the synaptic scaffolding protein PSD95. Our portfolio supports neuroscience and neurological disorder research with optimized monoclonal/polyclonal antibodies, antibody pairs, and ancillary reagents. For rare targets or custom requirements, CamelBio offers specialized sourcing and technical services to bridge the gap from concept to clinical application.