Anti-PSD95 Polyclonal Antibody for WB, IF/ICC, ELISA - P78352

Core Product Specifications and Parameters

Biological Background: PSD95 Function and Localization

• PSD95, encoded by the DLG4 gene, is a postsynaptic scaffolding protein essential for synaptogenesis and synaptic plasticity, interacting with NMDA receptors, potassium channels, and AMPA receptors.
• PSD95 is a member of the membrane-associated guanylate kinase (MAGUK) family, containing three PDZ domains, an SH3 domain, and a guanylate kinase-like domain, enabling multi-protein complex assembly.
• It localizes primarily to the postsynaptic density of excitatory synapses in the brain, with expression also detected in dendritic spines, axons, and presynaptic regions.
• PSD95 regulates AMPA receptor immobilization at the postsynaptic density, maintaining channels in an activated state to prevent synaptic depression (By similarity).
• It cooperates with FYN kinase to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane, preventing its endocytosis and thus ensuring proper palmitoylation of synaptic proteins. Related references: PMID:26334723
• Keywords and PTMs: alternative splicing; palmitoylation and phosphorylation regulate its synaptic targeting and function; disease-associated mutations are linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the SH3 domain region (aa 420–510); therefore, the antibody may recognize all isoforms containing this region.
• For Western blot, a primary band at approximately 80 kDa is expected in brain tissue lysates; additional bands may represent isoforms or degradation products.
• For immunofluorescence, optimal fixation and permeabilization conditions should be empirically determined to visualize synaptic localization.
• This polyclonal antibody detects human, mouse, and rat PSD95, making it suitable for cross-species studies; however, expression levels vary across tissues, so include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. As a trusted supplier to diagnostic manufacturers and research institutes, we streamline your sourcing from concept to clinic. This anti-PSD95 polyclonal antibody supports neuroscience research and synaptic target development, and our team can assist with customized rare target sourcing to accelerate your assay development.