Anti-PSD93/chapsyn-110/DLG2 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q15700

Core Product Specifications and Parameters

Biological Background: PSD93/chapsyn-110/DLG2 Function and Localization

• Disks large homolog 2 (DLG2), also known as PSD-93 or chapsyn-110, is a member of the membrane-associated guanylate kinase (MAGUK) family of scaffolding proteins.
• It functions as a critical postsynaptic scaffold protein at excitatory synapses, organizing receptor complexes and signaling molecules.
• DLG2 is required for the perception of chronic pain through NMDA receptor signaling, regulating surface expression of NMDA receptors in dorsal horn neurons of the spinal cord (by similarity).
• The protein interacts with cytoplasmic tails of NMDA receptor subunits and inward rectifying potassium channels, contributing to synaptic stability, particularly at cholinergic synapses.
• Subcellularly, DLG2 localizes to the cell membrane, postsynaptic density, synapse, and is found in cell projections such as axons and in the perikaryon.
• Post-translational modifications include palmitoylation, which anchors it to membranes, and phosphorylation, suggesting regulation of its scaffolding function.
• The protein contains multiple protein interaction domains, including PDZ, SH3, and guanylate kinase-like domains, enabling assembly of large signaling complexes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from an internal region (aa 600-700); therefore, the antibody is expected to recognize the target regardless of modifications at the N- or C-terminus.
• For Western blotting, a band at approximately 98 kDa is anticipated; consider using reducing conditions and validate in human, mouse, and rat cell lines known to express DLG2.
• In immunofluorescence-paraffin (IF-P) applications, antigen retrieval may enhance detection; test different fixation methods to preserve epitope accessibility.
• For ELISA, ensure the plate coating antigen matches the immunogen region or use full-length recombinant protein; perform cross-reactivity tests with related scaffolds if needed.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material provider, CamelBio streamlines your development process by offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PSD93/DLG2 antibody supports research into synaptic biology and pain signaling, and our sourcing experts can assist with custom requirements for rare or difficult targets, ensuring a seamless path from discovery to diagnostic application.