Anti-PSD2 Rabbit Polyclonal Antibody for WB and ELISA - Q9BQI7

Core Product Specifications and Parameters

Biological Background: PSD2 Function and Localization

• PSD2 (PH and SEC7 domain-containing protein 2), also known as EFA6C, is an exchange factor for ADP-ribosylation factor 6 (ARF6) and belongs to the cytohesin/ARNO family of guanine nucleotide exchange factors.
• Contains an N-terminal PH (pleckstrin homology) domain that mediates binding to phosphoinositides, promoting membrane association and localization to cellular membranes.
• The central SEC7 domain catalyzes GDP/GTP exchange on ARF6, a small GTPase that regulates endocytic membrane trafficking, actin cytoskeleton remodeling, and cell migration.
• Localizes to the cell membrane, ruffle membranes, and the cleavage furrow during cytokinesis, indicating functional roles in cell movement and division.
• Features a coiled-coil region and a transmembrane helix, which may facilitate protein oligomerization and stable membrane anchoring.
• Identified as a phosphoprotein, suggesting its activity is modulated by phosphorylation in response to upstream signaling pathways.

Experimental Guidance and Technical Tips

• For Western blotting, use reducing and denaturing conditions; the antibody is expected to detect a band at approximately 85 kDa in total cell lysates from human, mouse, or rat origin.
• Since the immunogen spans amino acids 1–190 (N-terminal PH domain region), the antibody may recognize both full-length PSD2 and potential N-terminal fragments—validate specificity in your sample system.
• In ELISA applications, standard indirect ELISA protocols can be applied; consider using the recombinant immunogen as a positive control to ensure assay performance.
• As a polyclonal antibody, verify batch-to-batch consistency for critical quantitative assays.
• Store lyophilized or concentrated antibody at -20°C; avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a streamlined path from concept to clinic through comprehensive IVD raw material supply. For ARF6 signaling and membrane trafficking research, we offer this anti-PSD2 polyclonal antibody together with validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets. Rely on CamelBio as your single partner for procurement efficiency and technical support.