Anti-PRX Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BXM0

Core Product Specifications and Parameters

Biological Background: Periaxin Function and Localization

• Periaxin is a scaffolding protein encoded by the PRX gene, with two main isoforms (isoform 1 and isoform 2) detected in sciatic nerve and Schwann cells.
• It functions as a critical component of the dystroglycan complex in Schwann cells, maintaining the peripheral myelin sheath essential for nerve impulse transmission and sensory perception.
• Periaxin is required for MBP mRNA transport, Schwann cell elongation, internode length regulation, and formation of Cajal bands and Schmidt-Lanterman incisures.
• It recruits DRP2 to the Schwann cell plasma membrane and contributes to the formation of appositions between the myelin sheath and the Schwann cell membrane.
• In the eye lens, periaxin interacts with EZR and AHNAK complexes, influencing fiber cell membrane protein composition and morphology.
• Subcellular localization includes the cell membrane, cytoplasm, nucleus, and cell junctions, reflecting its versatile scaffolding roles.
• Post-translational modifications include phosphorylation, as indicated by proteomic studies, and the protein is linked to Charcot-Marie-Tooth disease type 4F and Dejerine-Sottas syndrome.
• Tissue expression is prominent in the spinal cord and sciatic nerve, with high levels in Schwann cells.

Experimental Guidance and Technical Tips

• The immunogen is designed against a sequence within amino acids 1250-1350 of human PRX, targeting a region likely to detect both major isoforms; consider verifying isoform specificity if needed.
• For Western blot (WB), the predicted molecular weight is ~155 kDa; use appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/ICC (IF/ICC), expect staining at cell membranes, cytoplasm, and possibly nuclei in Schwann cell or lens fiber cell models; optimize permeabilization and fixation protocols.
• ELISA applications may require careful titration due to the polyclonal nature; cross-reactivity with mouse and rat orthologs should be confirmed in the specific assay setup.
• When working with nerve tissues or primary Schwann cells, include positive controls such as rodent sciatic nerve lysates to validate detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable access to this anti-PRX polyclonal antibody, targeting a key player in myelin biology and peripheral neuropathies. As a dedicated IVD raw material partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your development from concept to clinic. Our supply capabilities ensure you can secure consistent, high-quality materials for neuroscience and neuromuscular disease research.