Anti-PRSS8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16651

Core Product Specifications and Parameters

Biological Background: PRSS8 Function and Localization

• Prostasin (PRSS8) is a serine protease encoded by the PRSS8 gene, also known as channel-activating protease 1 (CAP1). It is a member of the trypsin-like serine protease family.
• It possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates.
• PRSS8 stimulates the epithelial sodium channel (ENaC) activity through proteolytic cleavage of the gamma subunit (SCNN1G), playing a critical role in sodium homeostasis.
• The protein is expressed in various tissues including prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus, and renal proximal tubular cells. In the prostate, it may be synthesized in epithelial cells and secreted into seminal fluid.
• Subcellular localization includes the cell membrane and extracellular space; it can be secreted, consistent with its role as a channel-activating protease.
• Post-translational modifications include glycosylation and disulfide bond formation; it is synthesized as a zymogen and requires proteolytic activation.
• Alternative splicing of PRSS8 generates multiple isoforms, which may have distinct regulatory roles.
• Relevant UniProt keywords: 3D-structure, Alternative splicing, Cell membrane, Direct protein sequencing, Disulfide bond, Glycoprotein, Hydrolase, Membrane, Protease, Proteomics identification, Reference proteome, Secreted, Serine protease, Signal, Transmembrane, Transmembrane helix, Zymogen.

Experimental Guidance and Technical Tips

• The antibody's immunogen corresponds to a peptide within the C-terminal region of human PRSS8 (amino acids 200 to the end). Researchers may consider this when assessing isoform specificity.
• For Western blotting, the predicted molecular weight of PRSS8 is approximately 36 kDa; validate the band pattern in the relevant sample system under reducing and non-reducing conditions.
• In immunofluorescence/ICC applications, appropriate fixation and permeabilization protocols should be optimized for the target cell type, given the protein's membrane and secreted localization.
• As the antibody shows reactivity with human, mouse, and rat PRSS8, it is suitable for cross-species studies; however, verify in the specific experimental context.
• For ELISA, pairing with a suitable detection system and validating against known standards in the matrix of interest is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting diagnostic manufacturers and researchers in epithelial biology and ion channel studies. This anti-PRSS8 antibody reflects our commitment to providing high-quality tools for ENaC-related research and assay development. For customized sourcing of rare targets or technical consultation, contact our team.