Anti-PRSS23 Polyclonal Antibody (Rabbit pAb) for WB, IHC-P, ELISA - O95084

Core Product Specifications and Parameters

Biological Background: PRSS23 Function and Localization

• PRSS23 (Serine protease 23), also known as SIG13, SPUVE, or ZSIG13, is a member of the serine protease family predicted to have proteolytic activity.
• The protein contains a signal peptide and is annotated as a secreted protein based on its subcellular location (UniProt: O95084).
• It features key structural motifs including disulfide bonds and potential glycosylation and phosphorylation sites, as indicated by the keywords "Disulfide bond", "Glycoprotein", and "Phosphoprotein".
• Alternative splicing of the PRSS23 gene gives rise to multiple transcript variants, expanding potential isoform diversity.
• PRSS23 is classified under the hydrolase class, consistent with its putative catalytic function.
• As a serine protease, it may be involved in extracellular matrix remodeling, cell signaling, or other proteolytic cascades, though its precise biological role remains to be fully characterized.
• The UniProt entry is a reviewed Swiss-Prot record, confirming it as a reference proteome member with experimental proteomics identification evidence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 101-220 of human PRSS23, a region likely to be extracellular and proximal to the catalytic domain. For detection of endogenous PRSS23, consider using a protease inhibitor cocktail during sample preparation to minimize degradation.
• Based on the provided applications (WB, IHC-P, ELISA), the antibody may be suitable for detecting PRSS23 in various sample types. For WB, the predicted molecular weight is approximately 43 kDa; however, post-translational modifications such as glycosylation may cause band shifts.
• In IHC-P, tissue fixation and antigen retrieval conditions should be optimized, and appropriate controls (e.g., blocking peptide) are recommended to confirm specificity.
• Cross-reactivity has been observed with human, mouse, and rat, allowing potential multi-species studies. Validate the antibody's performance in your specific experimental system to ensure consistent results.

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CamelBio streamlines the procurement of IVD raw materials for diagnostic manufacturers and research laboratories, from discovery to commercialization. Our portfolio encompasses validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—empowering you to accelerate assay development for serine protease targets like PRSS23. Let us be your single partner for reliable, high-quality raw materials and technical support.