Anti-PRSS2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P07478

Core Product Specifications and Parameters

Biological Background: Trypsin-2 Function and Localization

• Trypsin-2 (encoded by PRSS2) is an anionic serine protease, also known as serine protease 2 or trypsin II, belonging to the chymotrypsin/trypsin family of digestive enzymes.
• The protein is synthesized as an inactive zymogen (trypsinogen) with a signal peptide and a propeptide that is cleaved for activation in the small intestine.
• Key structural features include a serine protease catalytic triad (His, Asp, Ser), multiple disulfide bonds, and a calcium-binding site essential for stability.
• In the ileum, trypsin-2 is secreted by Paneth cells at the base of small intestinal crypts and participates in processing antimicrobial peptides, such as DEFA5 (defensin-5).
• Subcellular localization is extracellular; once activated, trypsin-2 acts in the intestinal lumen to degrade dietary proteins.
• Post-translational modifications include tyrosine sulfation and proteolytic removal of the activation peptide.
• According to UniProt keywords, trypsin-2 is involved in digestion, binds calcium, and has been identified by direct protein sequencing and proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human PRSS2; this N-terminal region includes part of the signal peptide and propeptide, which may influence antibody specificity for the precursor and mature forms.
• For Western blotting (WB), trypsin-2 has a predicted molecular weight of ~26 kDa; use appropriate positive controls like Paneth cell lysates or intestinal tissue extracts.
• In immunohistochemistry (IHC-P), validate staining in human, mouse, or rat small intestine sections, expecting cytoplasmic and secreted signals in Paneth cells.
• For ELISA, consider pairing this antibody as a capture/detection reagent against recombinant trypsin-2 or native samples; cross-reactivity with other trypsin isoforms should be evaluated.
• Given cross-reactivity listed for human, mouse, and rat, verify reactivity in each species using appropriate samples before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to accelerate diagnostic development. For PRSS2/trypsin-2 research, we provide reliable monoclonal antibodies and bulk-supply options suitable for immunoassays targeting digestive enzymes and Paneth cell function. Count on CamelBio's integrated procurement network to access rare targets and streamline your project from concept to clinic.