Anti-PRSS12 Rabbit Polyclonal Antibody for WB, ELISA - P56730

Core Product Specifications and Parameters

Biological Background: Neurotrypsin Function and Localization

• Neurotrypsin (PRSS12), also known as motopsin or leydin, is a member of the serine protease family characterized by an N-terminal signal peptide and multiple kringle domains.
• It functions as a secreted protease that modulates neuronal plasticity, putatively through proteolytic remodeling of synaptic structures to facilitate learning and memory processes.
• The protein is selectively expressed in the brain and in Leydig cells of the testis, consistent with its roles in cognitive function and reproductive biology.
• Classified as a hydrolase and serine protease, neurotrypsin contains conserved catalytic residues and undergoes post-translational modifications including glycosylation and disulfide bond formation.
• Mutations in the PRSS12 gene have been linked to intellectual disability, highlighting the essential role of neurotrypsin in cognitive development.
• The mature protein has a predicted molecular weight of approximately 97 kDa.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 21-300 of human PRSS12, encompassing the N-terminal region that includes the signal peptide and kringle domains; this region is expected to be accessible in the secreted protein.
• For Western blot analysis, consider using brain tissue lysates from mouse or rat, given the reported cross-reactivity, and validate specificity with appropriate controls such as knockout tissue or peptide competition.
• In ELISA applications, the antibody may be utilized for detection of native or recombinant neurotrypsin in cell culture supernatants or biological fluids, taking into account the secreted nature of the target.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays; perform pilot experiments to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, streamlining your project from early development to clinical application. This anti-neurotrypsin polyclonal antibody supports neuroscience and cognitive disorder research, backed by our robust supply chain for ancillary reagents and custom assay development solutions. With a commitment to rare target sourcing and bulk material provision, CamelBio serves as your agile partner for all raw material needs.