Anti-PRSS1 Polyclonal Antibody for WB, IHC-P, ELISA - P07477

Core Product Specifications and Parameters

Biological Background: PRSS1 Function and Localization

• PRSS1 encodes serine protease 1, a member of the trypsin family of digestive enzymes, also known as cationic trypsinogen, trypsin-1, or anionic trypsin I.
• The protein is secreted as an inactive zymogen (trypsinogen) and is activated by enteropeptidase in the small intestine, where it cleaves peptide bonds at the carboxyl side of basic amino acids such as arginine and lysine.
• Functional studies show activity against synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec, and Boc-Val-Pro-Arg-Mec, with the single-chain form being more active than the two-chain form.
• It contains disulfide bonds and binds calcium, which stabilizes the enzyme and prevents autolysis, as indicated by structural studies.
• Subcellular localization is primarily extracellular, being secreted into the intestinal lumen and extracellular space to perform its proteolytic function in digestion.
• The protein is classified under keywords such as secreted, serine protease, zymogen, disulfide bond, calcium, metal-binding, digestion, and hydrolase.
• PRSS1 is distinct from other trypsin isoforms (e.g., PRSS2, PRSS3) but shares significant sequence homology; it is predominantly expressed in the pancreas and is a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 24–247 of human PRSS1 (mature trypsin region), so the antibody may recognize both proenzyme (trypsinogen) and active trypsin forms; consider this when designing activation-state studies.
• For Western blot (WB), the predicted band size is approximately 24 kDa; however, observe that post-translational modifications (e.g., sulfation) or zymogen processing may cause slight shifts.
• In immunohistochemistry (IHC-P), note that PRSS1 is highly abundant in the pancreas; validate the antibody in appropriate positive (e.g., pancreas) and negative (e.g., non-secretory tissues) controls to assess specificity.
• Cross-reactivity is reported for human and rat; for experiments involving mouse or other species, verify reactivity independently due to potential sequence differences among trypsin isoforms.
• For ELISA applications, the polyclonal nature of the antibody allows capture or detection of PRSS1 in biological fluids such as serum or pancreatic juice; however, consider dilution optimization and interference from other trypsin-like proteases.
• Given the high sequence similarity within the trypsin family, evaluate possible cross-reactivity with PRSS2 or PRSS3 if working with samples where multiple isoforms may be present.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized mono- and polyclonal antibodies, and bulk ancillary reagents. For PRSS1-related research or diagnostic development, we can assist in sourcing tailored reagents targeting serine proteases or rare targets within the proteolytic enzyme family. Our team supports every stage from concept to clinic, enabling you to focus on assay development with confidence.