Anti-PRRX2 Rabbit Polyclonal Antibody for WB, ELISA - Q99811

Core Product Specifications and Parameters

Biological Background: PRRX2 Function and Localization

• PRRX2 (Paired mesoderm homeobox protein 2), also known as Paired-related homeobox protein 2 (PRX-2), is a homeobox transcription factor encoded by the PRRX2 gene (aliases: PMX2, PRX2).
• It functions as a DNA-binding developmental regulator, potentially involved in scarless healing of cutaneous wounds during early gestation.
• The protein localizes to the nucleus, in line with its transcriptional regulatory role.
• In fetal skin, PRRX2 is predominantly expressed in mesoderm-derived cells of the dermal papilla of developing hair follicles and is absent from the epidermis and dermis.
• In adult skin, it shows weak expression restricted to the basal layer of the epidermis, with no dermal expression.
• Keywords associated with PRRX2 include 3D-structure, developmental protein, DNA-binding, homeobox, nucleus, proteomics identification, and reference proteome.
• The calculated molecular weight is approximately 27 kDa (27079 Da).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 180–253 of human PRRX2 (NP_057391.1); verify sequence homology with mouse and rat orthologs if cross-species detection is critical.
• For Western blot (WB), the predicted molecular weight is ~27 kDa; optimize sample preparation and antibody dilution using relevant positive controls.
• ELISA applications may require titration of the antibody and careful selection of coating antigen; consider pairing with a validated detection system.
• As a polyclonal antibody, lot-to-lot variability is possible; include internal reference standards in each assay.
• Cross-reactivity has been reported with human, mouse, and rat samples; validate specificity in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by offering a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. The PRRX2 antibody product is ideal for developmental biology and wound healing studies, and CamelBio can assist with sourcing rare target reagents and providing technical support throughout assay development.