Anti-PRRX1 Rabbit Polyclonal Antibody for WB and ELISA - P54821

Core Product Specifications and Parameters

Biological Background: PRRX1 Function and Localization

• As a master transcription factor of stromal fibroblasts, PRRX1 drives myofibroblastic lineage progression and wound healing by remodeling super-enhancer landscapes in response to TGF-beta signaling, playing an essential role in tissue repair. Related references: PMID:35589735
• PRRX1 acts as a transcriptional regulator of muscle creatine kinase (MCK), influencing the establishment of diverse mesodermal muscle types and contributing to muscle development and function.
• It may also participate in the homeostasis and regeneration of bone, white adipose tissue, and dermis, indicating broader roles in mesenchymal tissue maintenance.
• The protein localizes to the nucleus, where it performs its transcriptional regulatory functions.
• PRRX1 is subject to post-translational modifications, including acetylation and phosphorylation, which may modulate its activity and stability.
• Two major isoforms exist due to alternative splicing, with isoform 1 predominating in most embryonic tissues and isoform 2 showing higher expression in skeletal muscle and heart; both are widely expressed in adults.
• Mutations in PRRX1 are associated with disease variants, underscoring its developmental importance.

Experimental Guidance and Technical Tips

• When performing Western blot, consider the predicted molecular weight of 27 kDa; however, due to alternative splicing or post-translational modifications such as acetylation and phosphorylation, the observed band may migrate differently. Validate detection in the relevant sample system.
• For ELISA, the antibody may be used for direct detection or as part of a sandwich pair. Optimization of coating concentration and blocking conditions is recommended to achieve optimal signal-to-noise ratios.
• Given that the immunogen corresponds to the N-terminal region (aa 1-90), this antibody may recognize both isoforms; however, users should verify specificity in their experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PRRX1 polyclonal antibody supports studies in transcriptional regulation, myofibroblast differentiation, and wound healing. Through our comprehensive supply network, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.