Anti-PRR5 Polyclonal Antibody for WB, IF/ICC, ELISA - P85299

Core Product Specifications and Parameters

Biological Background: PRR5 Function and Localization

• PRR5 (Protor-1) is a proline-rich protein that serves as an associated subunit of the mTORC2 complex, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors but is nutrient-insensitive, unlike mTORC1. Related references: PMID:17461779, PMID:17599906, PMID:29424687
• The mTORC2 complex functions upstream of Rho GTPases to regulate the actin cytoskeleton, likely by activating Rho-type guanine nucleotide exchange factors. Related references: PMID:17461779, PMID:17599906, PMID:29424687
• PRR5 modulates platelet-derived growth factor (PDGF) signaling by regulating PDGFRB expression. Related references: PMID:17599906
• PRR5 may function as a tumor suppressor in breast cancer. Related references: PMID:15718101
• PRR5 is predominantly expressed in kidney and liver, with high expression also in brain, spleen, testis, and placenta. Overexpression has been observed in colorectal tumors.
• PRR5 undergoes alternative splicing and is phosphorylated; it is involved in cell cycle regulation.

Experimental Guidance and Technical Tips

• The immunogen, spanning amino acids 190–388, targets a conserved region, suggesting potential cross-reactivity with mouse and rat PRR5.
• In Western blot, an expected band at approximately 43 kDa should be validated using lysates from PRR5-expressing tissues such as kidney or liver.
• For immunofluorescence, consider fixation conditions that preserve native protein interactions, as PRR5 is part of the mTORC2 complex.
• ELISA can be used to quantify PRR5 levels; appropriate standard curves and pairing antibodies should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for mTOR signaling research. Whether you need PRR5 antibodies for diagnostic assay development or rare target raw-material sourcing, our integrated supply chain ensures seamless procurement from concept to clinic.