Anti-PRR16 Rabbit Polyclonal Antibody for WB, ELISA - Q569H4

Core Product Specifications and Parameters

Biological Background: PRR16 Function and Localization

• PRR16, also known as Largen or DSC54, is a human protein encoded by the PRR16 gene (UniProt: Q569H4).
• It functions as a regulator of cell size, promoting cell size increase independently of mTOR and Hippo signaling pathways.
• PRR16 stimulates the translation of specific mRNAs, including those encoding proteins affecting mitochondrial functions.
• This leads to increased mitochondrial mass and respiration, playing a role in cellular metabolism.
• The protein is characterized by a coiled-coil domain and undergoes alternative splicing (UniProt keywords: Alternative splicing, Coiled coil).
• It has been identified in proteomics studies as a reference proteome component (UniProt keyword: Proteomics identification, Reference proteome).
• No specific subcellular localization or tissue specificity is reported in the current UniProt entry.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from human PRR16 (aa 50–150), ensuring specificity for the target.
• For Western blot (WB), consider testing in human and mouse cell lysates due to the reported mouse cross-reactivity; validate with appropriate controls.
• ELISA may be used for quantitative detection; optimize coating conditions and antibody dilutions for the specific assay format.
• As a polyclonal antibody, batch-to-batch variability may occur; include internal reference standards in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined sourcing solution for IVD raw materials, including validated antibodies like this anti-PRR16 polyclonal antibody for cell biology and metabolism studies. Beyond this product, we supply optimized monoclonal/polyclonal antibodies, recombinant proteins, and bulk ancillary reagents to support your assay development. Our one-stop services cover from concept to clinic, ensuring reliable supply for your diagnostic needs.