Anti-PRPS2 Polyclonal Antibody for WB, ELISA - P11908

Core Product Specifications and Parameters

Biological Background: PRPS2 Function and Localization

• PRPS2 (Ribose-phosphate pyrophosphokinase 2), also known as PPRibP and PRS-II, is a member of the ribose-phosphate pyrophosphokinase family.
• It catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) from ATP and ribose 5-phosphate, a critical step in nucleotide biosynthesis.
• PRPP is a key substrate for purine, pyrimidine, and pyridine nucleotide synthesis, linking carbohydrate metabolism to nucleotide production.
• The enzyme requires magnesium ions and ATP for its activity, functioning as both a kinase and transferase.
• Alternative splicing of the PRPS2 gene generates multiple transcript variants, potentially diversifying its regulation.
• Structural studies have revealed a conserved 3D fold, and the protein has been identified in multiple proteomics screens.
• The predicted molecular weight is approximately 34.8 kDa (UniProt), consistent with the 35 kDa band observed in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–240) of human PRPS2; consider this when designing detection experiments for endogenous or recombinant protein forms.
• For Western blot, a specific band around 35 kDa is expected; validation in your sample panel (e.g., human, rat cell lines) is recommended.
• In ELISA, this antibody may serve as a capture or detection reagent; optimize coating concentrations and antibody dilutions under your assay conditions.
• The product cross-reacts with human and rat PRPS2; reactivity with other species should be verified empirically.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material partner, CamelBio supports diagnostic manufacturers and researchers with validated antibodies, antigen pairs, and ancillary reagents for nucleotide metabolism targets like PRPS2. Whether you need bulk polyclonal antibodies, matched monoclonal pairs, or custom rare-target sourcing, CamelBio delivers integrated, clinic-to-market solutions to accelerate your development pipeline.