Anti-PRPS2 Polyclonal Antibody for WB, ELISA - P11908

Core Product Specifications and Parameters

Biological Background: PRPS2 Function and Localization

• PRPS2 encodes ribose-phosphate pyrophosphokinase 2, also referred to as phosphoribosyl pyrophosphate synthase II (PRS-II) or PPRibP.
• The enzyme catalyzes the Mg²⁺-dependent conversion of ATP and ribose 5-phosphate into phosphoribosylpyrophosphate (PRPP), a key precursor for de novo purine and pyrimidine nucleotide synthesis.
• Functional annotation classifies PRPS2 as a kinase and transferase, highlighting its critical role in nucleotide metabolism.
• The protein contains conserved ATP- and metal-binding motifs, with magnesium serving as an essential cofactor for catalytic activity.
• Alternative splicing of the PRPS2 gene generates multiple transcript variants, potentially modulating enzyme activity or regulation.
• The predicted molecular mass of PRPS2 is ~34.8 kDa, aligning with the observed 35 kDa band in Western blotting.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering the N-terminal region (aa 1-240) of human PRPS2; it may recognize full-length and truncated isoforms in lysates.
• For Western blotting, a band around 35 kDa is expected in human, mouse, and rat samples; consider using appropriate positive controls (e.g., HEK293, HeLa, liver tissue).
• ELISA can be employed to quantify PRPS2 levels or screen for antigen-antibody interactions; validate the assay conditions with recombinant PRPS2 protein.
• Cross-reactivity with other PRPS family members (e.g., PRPS1) cannot be excluded; verify specificity using isoform-specific controls or knockout cell lines.
• Optimize blocking and antibody dilution conditions for each application and species, as the polyclonal nature may lead to batch-to-batch variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For nucleotide metabolism research, our PRPS2 Rabbit pAb serves as a reliable tool for WB and ELISA, backed by efficient one-stop sourcing services from concept to clinic. Leverage our rare-target sourcing capabilities to accelerate your diagnostic development pipeline.