Anti-PRPH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P41219

Core Product Specifications and Parameters

Biological Background: Peripherin (PRPH) Function and Localization

• Peripherin is a class-III neuronal intermediate filament protein, also known as Neurofilament 4 (NEF4).
• It forms an independent structural network or cooperates with other neurofilament proteins (NEFL, NEFH, NEFM, INA) to form filamentous networks; assembly may be regulated by RAB7A. Related references: PMID:15322088 PMID:15446584
• PRPH plays a role in the development of unmyelinated sensory neurons and may be involved in axon elongation and regeneration after injury.
• It inhibits neurite extension in type II spiral ganglion neurons in the cochlea.
• Subcellularly, peripherin localizes to the cytoplasm, cytoskeleton, axon, and perikaryon.
• Expressed predominantly in neurons of the outer hair cells in the organ of Corti and to a lesser extent in type I spiral ganglion cells.
• Post-translational modifications include phosphorylation and nitration; implicated in amyotrophic lateral sclerosis and neurodegeneration.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 54 kDa. Validate antibody specificity using PRPH-positive neuronal cell lines or tissue lysates.
• For IF/ICC, optimize fixation and permeabilization conditions to preserve intermediate filament integrity; co-staining with other cytoskeletal markers is recommended.
• For ELISA, use recombinant full-length or fragment PRPH protein as a reference standard; titrate antibody for optimal signal-to-noise ratio.
• Cross-reactivity with mouse and rat enables applications in rodent models of peripheral neuropathy and neurodegeneration.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material supplier, CamelBio offers comprehensive access to antibodies, antigen pairs, and ancillary reagents for neuroscience and neurodegeneration research. For PRPH-targeted studies, we provide validated polyclonal and monoclonal antibodies, bulk quantities, and custom sourcing for rare intermediate filament targets. Let CamelBio bridge your diagnostic development from concept to clinic with reliable, high-quality raw materials.