Anti-PRPF8 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6P2Q9

Core Product Specifications and Parameters

Biological Background: PRPF8 Function and Localization

• PRPF8 (Pre-mRNA-processing-splicing factor 8) is also known as PRP8 homolog, 220 kDa U5 snRNP-specific protein, and p220, encoded by the PRPF8 gene in humans.
• It functions as a core component of the precatalytic, catalytic, and postcatalytic spliceosomal complexes in both the major U2-type and minor U12-type spliceosomes, playing a critical role in pre-mRNA splicing. Related references: PMID:10411133 PMID:11971955 PMID:28076346
• Acts as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs, and is required for the formation of the U4/U6-U5 tri-snRNP complex.
• Positions spliceosomal U2, U5, and U6 snRNAs at splice sites on pre-mRNA substrates and interacts with both the 5' and 3' splice sites to facilitate splicing.
• Localizes to the nucleus, particularly in nuclear speckles, which are sites of pre-mRNA processing.
• Widely expressed across tissues, indicating its fundamental role in gene expression.
• Subject to post-translational modifications including acetylation, methylation, and phosphorylation, which may regulate its activity.
• Associated with retinitis pigmentosa, a disease variant, underscoring its clinical significance.

Experimental Guidance and Technical Tips

• For western blot (WB), note that PRPF8 has a predicted molecular weight of 274 kDa; use a low-percentage SDS-PAGE gel (e.g., 6–8%) and optimize transfer conditions for high-molecular-weight proteins.
• The immunogen covers amino acids 2200–2335, suggesting recognition of a C-terminal epitope. Consider this if using truncated constructs.
• Cross-reactivity is shown with human, mouse, and rat; validate in your specific sample system.
• Applications listed include WB, IHC-P, IF/ICC, and ELISA; optimize dilutions and conditions for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With this PRPF8 rabbit monoclonal antibody, we support your splicing research and diagnostic assay development. Our expert team can also assist in rare target raw-material sourcing to accelerate your project from concept to clinic.