Anti-PRPF4B Rabbit Polyclonal Antibody for WB and ELISA - Q13523

Core Product Specifications and Parameters

Biological Background: PRPF4B Function and Localization

• PRPF4B is a serine/threonine kinase involved in spliceosomal assembly, mitosis, and signaling regulation. Related references: PMID:10799319 PMID:12077342 PMID:17513757
• It connects chromatin-mediated transcriptional regulation with pre-mRNA splicing by associating with the U5 snRNP and NCOR1 deacetylase complexes. Related references: PMID:12077342
• PRPF4B phosphorylates PRPF6 and PRPF31 to promote spliceosome B complex formation.
• It phosphorylates SRSF1, a key splicing factor. Related references: PMID:11418604
• During mitosis, PRPF4B localizes to kinetochores and is essential for recruiting and maintaining checkpoint proteins MAD1L1 and MAD2L1. Related references: PMID:17998396
• PRPF4B regulates transcription factors such as ELK1 and KLF13 through phosphorylation. Related references: PMID:10799319 PMID:17513757
• It modulates Hippo signaling by phosphorylating YAP1 and WWTR1/TAZ, leading to their nuclear exclusion. Related references: PMID:29695716
• PRPF4B is predominantly nuclear, with localization to the centromere/kinetochore during mitosis.
• It is ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 550-760 of human PRPF4B (C-terminal region); the antibody is expected to recognize full-length PRPF4B and possibly isoforms containing this region.
• For Western blotting, use a low-percentage gel (e.g., 8%) to resolve the ~117 kDa protein, and include appropriate loading and molecular weight controls.
• Validate the antibody in samples from human, mouse, and rat, as cross-reactivity is reported.
• For ELISA applications, consider using the recombinant immunogen protein as a positive control to establish assay conditions.
• Due to the polyclonal nature, lot-to-lot variability may occur; it is advisable to titrate the antibody for optimal performance in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, covering every stage from concept to clinic. With expertise in kinases, splicing, and mitosis research, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your diagnostic or research projects. Trust CamelBio for reliable, scalable supply solutions.