Anti-PRPF4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43172

Core Product Specifications and Parameters

Biological Background: PRPF4 Function and Localization

• PRPF4, also known as PRP4 homolog, U4/U6 snRNP 60 kDa protein, or WD splicing factor Prp4, is a component of the U4/U6 small nuclear ribonucleoprotein (snRNP) particle.
• It functions in pre-mRNA splicing as part of the U4/U6-U5 tri-snRNP complex, which is essential for spliceosome assembly and formation of the precatalytic spliceosome (B complex).
• The protein contains WD repeat domains, which facilitate protein-protein interactions within the spliceosomal machinery.
• PRPF4 localizes predominantly to the nucleus, where it is enriched in nuclear speckles—subnuclear compartments associated with mRNA processing.
• Mutations in the PRPF4 gene have been linked to retinitis pigmentosa, highlighting its critical role in cellular RNA processing.
• The molecular mass of PRPF4 is approximately 58 kDa, consistent with its 58.4 kDa predicted weight.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–260) of human PRPF4; this region may be conserved across species, contributing to the observed cross-reactivity with mouse and rat.
• For Western blot, a band near 58 kDa is expected; consider optimizing lysis and loading conditions for your cell or tissue type.
• In immunohistochemistry (IHC-P), validate with positive and negative control tissues; antigen retrieval methods may influence staining intensity.
• ELISA applications may employ the antibody as a capture or detection reagent; titrate to determine optimal working concentrations.
• Because PRPF4 is a splicing factor, its expression levels may vary between proliferating and differentiated cells; account for this in experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with high-quality antibodies, validated pairs, and bulk ancillary reagents. This PRPF4 polyclonal antibody is well-suited for studies of spliceosome biology and splicing-related disorders. Beyond individual antibodies, CamelBio offers streamlined procurement of rare-target reagents, customizable antibody development, and technical consulting to accelerate your project from concept to clinic.