Anti-PRPF3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - O43395

Core Product Specifications and Parameters

Biological Background: PRPF3 Function and Localization

• PRPF3 encodes the U4/U6 small nuclear ribonucleoprotein Prp3, also known as pre-mRNA-splicing factor 3 (hPrp3) and U4/U6 snRNP 90 kDa protein.
• It is a core component of the U4/U6-U5 tri-snRNP complex, which is essential for spliceosome assembly during pre-mRNA splicing.
• The protein functions within both the tri-snRNP and the precatalytic spliceosome (B complex), facilitating the recognition and removal of introns.
• PRPF3 localizes to the nucleus, with enrichment in nuclear speckles where splicing factors are concentrated.
• Expression is highest in retina, liver, kidney, and blood, with lower levels detected in heart and brain.
• Post-translational modifications include phosphorylation and isopeptide bonds, as indicated by proteomics data.
• Mutations in PRPF3 are associated with retinitis pigmentosa, an inherited retinal degenerative disease.
• Alternative splicing of the PRPF3 transcript yields multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-280) of human PRPF3, suggesting the antibody may recognize full-length and N-terminal fragments.
• For Western blot, consider using whole cell lysates from human, mouse, or rat tissues; a band around 78 kDa is expected.
• Immunoprecipitation and IF/ICC may require optimization of fixation/permeabilization conditions due to nuclear localization.
• Validate the antibody in the relevant sample system and appropriate positive controls (e.g., retinal or liver tissues where PRPF3 is highly expressed).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-PRPF3, and bulk ancillary reagents. Our expertise spans rare target sourcing and technical consulting, supporting your spliceosome research and diagnostic development from concept to clinic.