Anti-PRP6 Rabbit Polyclonal Antibody for WB, ELISA - O94906

Core Product Specifications and Parameters

Biological Background: PRPF6 Function and Localization

• PRPF6 (Pre-mRNA-processing factor 6) is a core component of the U4/U6-U5 tri-snRNP complex, which assembles into the spliceosome to catalyze pre-mRNA splicing. Related references: PMID:20118938 PMID:21549338 PMID:28781166
• Known aliases include PRP6 homolog, U5-102 kDa protein (U5-102K), and androgen receptor N-terminal domain-transactivating protein 1 (ANT-1).
• Beyond splicing, it acts as a transcriptional coactivator by enhancing androgen receptor (AR) and glucocorticoid receptor (NR3C1) transactivation in response to dihydrotestosterone and dexamethasone, respectively, without affecting estrogen receptor activity.
• PRPF6 localizes primarily to the nucleoplasm and nuclear speckles, consistent with its roles in mRNA processing and gene regulation.
• The protein is subject to phosphorylation and alternative splicing, and contains repeated sequence motifs characteristic of spliceosomal proteins.
• Mutations in the PRPF6 gene are linked to retinitis pigmentosa, highlighting its importance in retinal cell function and splicing fidelity.
• PRPF6 is widely expressed across tissues, reflecting its fundamental role in constitutive and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 592–941) of human PRPF6, which may be conserved across splice variants; consider that the antibody might detect multiple isoforms or degradation products.
• For Western blot, use the predicted molecular weight of ~107 kDa as a reference, but be aware that phosphorylation and other post-translational modifications could cause shifts in migration.
• To confirm specificity, employ appropriate positive controls (e.g., HeLa or HEK293 whole-cell lysates) and negative controls (e.g., PRPF6 knockdown samples).
• For ELISA, optimize coating conditions with the recombinant immunogen and test a range of antibody concentrations to establish a robust standard curve.
• This antibody is validated for WB and ELISA; if other applications (e.g., IHC, IF) are desired, empirical optimization and validation in the relevant sample system are necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This anti-PRP6 polyclonal antibody is part of our extensive portfolio for pre-mRNA splicing and nuclear receptor research, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your diagnostic development pipeline.