Anti-PRP31 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8WWY3

Core Product Specifications and Parameters

Biological Background: PRPF31 Function and Localization

• PRPF31, also known as U4/U6 small nuclear ribonucleoprotein Prp31, is a 55 kDa RNA-binding protein that shares high homology across human, mouse, and rat.
• It is a core component of the spliceosome and essential for pre-mRNA splicing (PubMed:11867543, PubMed:20118938, PubMed:28781166).
  Related references: PMID:11867543, PMID:20118938, PMID:28781166
• PRPF31 is required for the assembly of the U4/U5/U6 tri-snRNP complex, a critical step in spliceosome formation (PubMed:11867543).
  Related references: PMID:11867543
• Localizes predominantly to the nucleus, with enrichment in nuclear speckles and Cajal bodies, compartments specialized for mRNA processing and maturation.
• Ubiquitously expressed across human tissues, consistent with its fundamental role in constitutive splicing.
• Post-translational modifications including acetylation, phosphorylation, and ubiquitination have been identified by mass spectrometry, potentially regulating its activity.
• Genetic mutations in PRPF31 are linked to autosomal dominant retinitis pigmentosa, making it a clinically relevant target for inherited retinal disease research.
• Contains characteristic coiled-coil domains and an RNA recognition motif, typical of spliceosomal proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–200) of human PRPF31; this sequence is conserved across species, which may explain the antibody's cross-reactivity with mouse and rat orthologs.
• In Western blot (WB), a predominant band near 55 kDa is expected; additional bands may arise from splice variants or post-translational modifications. Validate with positive control lysates (e.g., HeLa, HEK293) and consider loading controls.
• For immunohistochemistry (IHC-P), antigen retrieval (heat-induced with citrate buffer pH 6.0 or Tris-EDTA pH 9.0) is recommended; test on paraffin-embedded sections of tissues with high PRPF31 expression, such as tonsil or testis.
• ELISA applications may be optimized with direct or capture formats; typical starting dilutions range from 1:500 to 1:2000, but titration is essential.
• The antibody is suitable for detecting endogenous PRPF31 in human, mouse, and rat models, but researchers should confirm specificity via knockdown/out validation in their systems.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio streamlines the supply of IVD raw materials, including validated antibodies like this anti‑PRPF31 polyclonal antibody, for research into mRNA splicing and related spliceosomopathies. Beyond antibodies, we offer a comprehensive portfolio of recombinant proteins, antigen‑matched antibody pairs, and ancillary reagents to accelerate diagnostic kit development. For rare or custom targets such as other spliceosome components, our team can assist manufacturers and laboratories in sourcing or developing specialized raw materials.