Anti-PRP19 Polyclonal Antibody for WB - Q9UMS4

Core Product Specifications and Parameters

Biological Background: PRP19 Function and Localization

• PRP19 (Pre-mRNA-processing factor 19, also known as PSO4, SNEV, hPso4, NMP200, and UBOX4) is a ubiquitin-protein ligase and an essential spliceosome component required for pre-mRNA splicing. Related references: PMID:28076346, PMID:28502770, PMID:29301961
• As part of the PRP19C/Prp19 complex, it mediates Lys-63-linked polyubiquitination of U4 spliceosomal protein PRPF3, facilitating recognition by U5 component PRPF8 and stabilizing the U4/U5/U6 tri-snRNP complex. Related references: PMID:20595234
• PRP19 is recruited to RNA polymerase II C-terminal domain and pre-mRNA, coupling transcription with splicing machinery. Related references: PMID:21536736
• The XAB2 complex, which includes PRP19, also participates in pre-mRNA splicing, transcription, and transcription-coupled repair. Related references: PMID:17981804
• In the DNA damage response, PRP19 is recruited by the RPA complex to damage sites, where it directly ubiquitinates RPA1 and RPA2 with Lys-63-linked chains, promoting ATR-ATRIP complex recruitment and ATR activation. Related references: PMID:24332808
• It plays a role in DNA double-strand break repair by recruiting SETMAR to damaged DNA and may also participate in DNA interstrand crosslink repair. Related references: PMID:18263876, PMID:16223718
• PRP19 can also mediate Lys-48-linked polyubiquitination, targeting substrates for proteasomal degradation. Related references: PMID:11435423
• Subcellular localization includes the nucleus, nucleoplasm, cytoplasm, cytoskeleton (spindle), and lipid droplets; it is ubiquitously expressed but weakly in senescent cells and highly in tumor cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 127-416), enabling detection of full-length PRP19 and potentially relevant isoforms.
• For western blot (WB), a predominant band at approximately 55 kDa is expected; validate the signal in the relevant sample system, as expression levels vary with cellular senescence and tumor status.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval optimization and appropriate controls to account for the protein's nuclear and cytosolic distribution.
• The antibody's cross-reactivity with mouse and rat makes it suitable for comparative studies; however, verify reactivity in your experimental model.
• Given PRP19's roles in spliceosome and DNA repair pathways, this antibody can be used to investigate protein interactions and complex formation via immunoprecipitation (IP).
• For ELISA applications, determine the optimal coating concentration empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, supporting diagnostic developers from target validation to assay optimization. This anti-PRP19 polyclonal antibody facilitates research into pre-mRNA splicing and DNA damage response, and we can complement it with validated antibody pairs, bulk ancillary reagents, and customized procurement for rare targets. Our B2B expertise ensures a seamless supply chain, accelerating your journey from concept to clinic.