Anti-Protoporphyrinogen Oxidase (PPOX) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P50336

Core Product Specifications and Parameters

Biological Background: PPOX Function and Localization

• Protoporphyrinogen oxidase (PPOX) is a mitochondrial enzyme localized to the inner membrane, where it catalyzes the six-electron oxidation of protoporphyrinogen-IX to protoporphyrin-IX, a critical step in the heme biosynthesis pathway.
• PPOX is a flavoprotein that requires FAD (flavin adenine dinucleotide) as a cofactor for its enzymatic activity.
• Mutations in the PPOX gene are associated with variegate porphyria, an autosomal dominant disorder characterized by cutaneous photosensitivity and acute neurovisceral attacks.
• The protein is widely expressed across tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• PPOX belongs to the protoporphyrinogen oxidase family and shares structural features with other flavin-dependent oxidoreductases.

Experimental Guidance and Technical Tips

• For Western blot analysis, a band at approximately 51 kDa is expected for endogenous PPOX in human and mouse samples. Validate using positive controls such as liver or heart tissue lysates.
• In immunohistochemistry (paraffin), PPOX is mitochondrial; expect granular cytoplasmic staining. Optimal antigen retrieval may be required; consider testing heat-mediated retrieval with citrate buffer.
• For ELISA, the antibody can be used as a capture or detection reagent; assess coating conditions and detection range with recombinant PPOX protein.
• As an N‑terminal immunogen (aa 1–280) was used, the antibody may recognize potential cleavage products or isoforms containing this region; confirm specificity with recombinant protein controls.

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CamelBio provides diagnostic manufacturers and research institutions with reliable access to high-quality IVD raw materials, including polyclonal antibodies like this validated anti‑PPOX antibody for heme biosynthesis studies. Our comprehensive portfolio includes optimized antibody pairs, conjugated secondary reagents, and bulk enzyme formulations, complemented by sourcing support for rare targets and custom development services. Partner with us to accelerate your assay development from concept to clinic.