Anti-Prothrombin (F2) Rabbit Polyclonal Antibody for WB and ELISA - P00734

Core Product Specifications and Parameters

Biological Background: Prothrombin (F2) Function and Localization

• Prothrombin (coagulation factor II) is the zymogen precursor of thrombin, a serine protease that plays a central role in blood coagulation, hemostasis, and wound healing.
• Thrombin cleaves peptide bonds after arginine and lysine residues, converting soluble fibrinogen into insoluble fibrin to form blood clots.
• In addition to fibrinogen, thrombin activates coagulation factors V, VII, VIII, XIII, and, when bound to thrombomodulin, activates protein C, which exerts anticoagulant effects.
• Thrombin mediates activation of coagulation factor XI (F11), a process enhanced by negatively charged surfaces. Related references: PMID:2019570, PMID:21976677
• Thrombin also triggers pro-inflammatory responses in endothelial cells, inducing the production of cytokines such as MCP-1/CCL2 and IL8/CXCL8. Related references: PMID:30568593, PMID:9780208
• Prothrombin is primarily synthesized in the liver and secreted into the plasma, consistent with its systemic role in coagulation.
• The protein contains multiple structural domains, including kringle domains and a gamma-carboxyglutamic acid (GLA) domain that facilitates calcium-dependent membrane binding.
• Post-translational modifications such as gamma-carboxylation and disulfide bond formation are essential for its biological activity.
• Mutations in the F2 gene are associated with thrombophilia and bleeding disorders.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 364–622 of human prothrombin, targeting the thrombin heavy chain region; this design may allow detection of both prothrombin and activated thrombin fragments.
• For Western blotting, expect a major band at ~70 kDa for full-length prothrombin under reducing conditions; additional lower molecular weight bands may appear due to proteolytic processing.
• Because this is a polyclonal antibody, validate performance in your specific sample system (e.g., human plasma or recombinant protein) to confirm specificity and lot-to-lot consistency.
• For ELISA, the antibody may be used as a capture or detection reagent; however, pairing with a non-overlapping monoclonal antibody could improve sensitivity and reduce background.
• Optimal working dilutions and incubation conditions should be determined empirically for each application; published data or standard protocols can serve as a starting point.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing high-quality antibodies like this anti-prothrombin (F2) polyclonal antibody to support coagulation research and diagnostic assay development. We offer streamlined sourcing solutions for validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents—connecting your lab with the critical tools needed from concept to clinic.