Anti-Proteinase K Rabbit Polyclonal Antibody - P06873

Core Product Specifications and Parameters

Biological Background: Proteinase K Function and Localization

• Proteinase K is a broad-spectrum serine protease originally isolated from the fungus Parengyodontium album (formerly Tritirachium album), encoded by the PROK gene.
• It belongs to the subtilisin-like serine protease family and is characterized by the classical catalytic triad (Asp-His-Ser) responsible for peptide bond hydrolysis.
• The enzyme preferentially cleaves peptide bonds adjacent to aromatic and hydrophobic amino acid residues, enabling effective degradation of keratin and other recalcitrant proteins.
• Proteinase K is synthesized as a preproprotein with a signal peptide and a propeptide, which are proteolytically removed to yield the active mature enzyme of approximately 28.9 kDa (mature form) with a full preprotein molecular weight of around 40.3 kDa.
• Its stability and activity are enhanced by calcium binding, which protects against autolysis and thermal denaturation; two disulfide bonds further stabilize the tertiary structure.
• Three-dimensional structures have been resolved (e.g., PDB entries), revealing a typical subtilisin fold with a deep substrate-binding cleft.
• Due to its robust broad-spectrum proteolytic activity, Proteinase K is widely employed in molecular biology for protein removal during nucleic acid purification and in various industrial applications.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant Proteinase K protein, which may present both native and denatured epitopes; consider this when choosing between native and denaturing conditions for your assay.
• For WB, ensure proper sample preparation including appropriate lysis buffers and protease inhibitors to avoid target degradation. Use a suitable anti-rabbit secondary antibody and optimize blocking to reduce background.
• For ELISA, validate the assay with known positive and negative controls; the antibody’s reactivity with native vs. coated antigen may differ, so consider antigen immobilization strategies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes integrated access to IVD raw materials, covering every stage from concept to clinic. This Proteinase K rabbit polyclonal antibody is available as part of CamelBio’s portfolio, which includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials. Explore our catalog to streamline your procurement and accelerate your Proteinase K-related research and diagnostic development.