Anti-Protein C Monoclonal Antibody for WB, IF-P, ELISA - P04070

Core Product Specifications and Parameters

Biological Background: Protein C Function and Localization

• Protein C is a vitamin K-dependent serine protease that functions as a natural anticoagulant by inactivating coagulation factors Va and VIIIa, a process that requires calcium ions and phospholipids. Related references: PMID:25618265 PMID:39880037
• It also exerts a protective effect on the endothelial cell barrier, contributing to vascular integrity. Related references: PMID:25651845
• Synthesized predominantly in the liver, Protein C is secreted into the plasma and cycles through the Golgi apparatus and endoplasmic reticulum during processing.
• The protein contains gamma-carboxyglutamic acid (Gla) residues that mediate calcium-dependent binding to phospholipid membranes, essential for its anticoagulant function.
• Structurally, it includes EGF-like domains and a serine protease domain, similar to other vitamin K-dependent coagulation factors.
• Post-translational modifications include vitamin K-dependent carboxylation, proteolytic cleavage, and hydroxylation; it circulates as a zymogen that is activated by the thrombin-thrombomodulin complex.
• Mutations in PROC are associated with protein C deficiency, an inherited risk factor for thrombophilia and venous thromboembolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300–461 of human Protein C, a region within the serine protease domain; consider this when designing epitope-based assays.
• For Western blot (WB), a band around 52 kDa is expected under reducing conditions; validate in relevant sample systems such as liver lysates or plasma.
• In immunofluorescence (IF-P), Protein C can be detected in endothelial cells or liver tissue; optimize fixation and permeabilization protocols for secretory pathway proteins.
• For ELISA, note the antibody cross-reacts with mouse Protein C; standard curve and matrix validation in human and murine samples are recommended.
• This monoclonal antibody is compatible with multiple applications, but individual assay conditions (e.g., primary antibody dilution, incubation time) should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering this anti-Protein C monoclonal antibody alongside validated antibody pairs and bulk ancillary reagents for coagulation assay development. Whether you need optimized antibodies, reagent sourcing, or support for rare targets in hemostasis research, we bridge your procurement needs with high-quality supplies to streamline diagnostic manufacturing.