Anti-Proteasome 20S LMP7/PSMB8 Monoclonal Antibody for WB, IHC-P, ELISA - P28062

Core Product Specifications and Parameters

Biological Background: Proteasome 20S LMP7/PSMB8 Function and Localization

• PSMB8 encodes the proteasome subunit beta type-8, also known as low molecular mass protein 7 (LMP7), which is a component of the immunoproteasome.
• This subunit replaces the constitutive PSMB5 in the immunoproteasome, enhancing the capacity to cleave peptides after hydrophobic and basic residues, and plays a critical role in generating antigenic peptides for MHC class I presentation. Involved in the generation of spliced peptides. Related references: PMID:27049119
• PSMB8 is a key mediator of interferon gamma-induced sensitivity and participates in apoptosis by degrading the anti-apoptotic protein MCL1.
• It is required for the differentiation of preadipocytes into adipocytes, indicating non-immune functions.
• The protein localizes to both the cytoplasm and nucleus.
• Post-translational modifications include phosphorylation, and it functions as a threonine protease that is initially synthesized as a zymogen.
• Alternative splicing produces isoforms (E1 and E2) with distinct functions; in cancer cells, substitution of isoform 1 by isoform 2 results in immunoproteasome deficiency.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of PSMB8 is approximately 30 kDa; consider using a positive control lysate from cell lines or tissues known to express the immunoproteasome, such as IFN-γ-treated cells.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary to expose the epitope; optimize antibody dilution using a tissue known to express PSMB8, such as immune organs.
• For ELISA applications, the recombinant protein immunogen suggests suitability for both direct and sandwich formats; titrate the antibody to determine optimal working concentration.
• As a rabbit monoclonal antibody, expect high specificity and reproducibility; consider cross-adsorption if non-specific binding is observed in multispecies samples.
• Cross-reactivity with mouse and rat PSMB8 indicates potential for use in rodent models; however, confirm specificity in the target species' tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. From validated antibody pairs and recombinant proteins to specialized reagents for immunoproteasome research, we support your workflow from initial discovery to clinical application. Whether you require bulk quantities of this anti-PSMB8 monoclonal antibody or customized sourcing for rare targets, our team ensures reliable supply and technical consultation.