Anti-Prostatic Acid Phosphatase (PSAP) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P15309

Core Product Specifications and Parameters

Biological Background: Prostatic Acid Phosphatase Function and Localization

• Prostatic acid phosphatase (PSAP), also known as ACPP, 5'-NT, or ACP-3, is a non-specific tyrosine phosphatase encoded by the ACP3 gene in humans.
• Catalyzes dephosphorylation of various substrates under acidic conditions (pH 4–6), including alkyl, aryl, and acyl phosphate monoesters, and phosphorylated proteins. Related references: PMID:10506173, PMID:15280042, PMID:20498373
• Acts as a lipid phosphatase, inactivating lysophosphatidic acid in seminal plasma. Related references: PMID:10506173, PMID:15280042, PMID:20498373
• Functions as a tumor suppressor in prostate cancer by dephosphorylating ERBB2 and suppressing MAPK-mediated signaling. Related references: PMID:20498373
• Has ecto-5'-nucleotidase activity in dorsal root ganglion neurons, generating adenosine from AMP to modulate pain.
• In seminal plasma, PSAP forms amyloid beta-sheet fibrils (SEVI) that capture and enhance HIV infection by attaching virions to target cells. Related references: PMID:18083097, PMID:19451623, PMID:19897482
• Subcellularly localized to the cell membrane, lysosome membrane, secreted fraction, nucleus, and cytoplasm.
• Highly expressed in prostate epithelial cells; also detected in bladder, kidney, pancreas, lung, cervix, testis, ovary, and skeletal muscle sarcolemma.
• Post-translationally modified by glycosylation and stabilized by disulfide bonds; classified as a glycoprotein with a signal peptide and transmembrane region.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 33–386 of human PSAP, covering the majority of the mature protein; this suggests broad epitope coverage, but monoclonal specificity should be confirmed in your assay system.
• For Western blotting, validate detection of the ~45 kDa band and note that glycosylation may cause a slightly higher apparent molecular weight; consider using deglycosylation controls if needed.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions (e.g., heat-induced epitope retrieval in citrate or Tris-EDTA buffer) and test antibody dilution series on positive (prostate tissue) and negative controls.
• In ELISA, the antibody’s reactivity with human and rat PSAP may enable cross-species quantitative analysis; validate standard curves and assay specificity.
• Since this rabbit monoclonal antibody has been tested in WB, IHC-P, and ELISA, additional applications (e.g., IF, flow cytometry) should be piloted with appropriate fixatives and permeabilization methods.
• Store at –20°C upon receipt and avoid repeated freeze-thaw cycles; the product is shipped on ice bags to maintain stability.

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CamelBio is a trusted partner for diagnostic manufacturers and research laboratories, supplying validated IVD raw materials including monoclonal antibodies against cancer biomarkers such as PSAP. Our comprehensive catalog covers bulk reagents, ancillary materials, and rare target sourcing to support your assay development from concept to clinical application. Contact our technical team for custom antibody panel solutions and seamless procurement for prostate cancer or infectious disease studies.