Anti-Prostatic Acid Phosphatase (ACPP) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P15309

Core Product Specifications and Parameters

Biological Background: Prostatic Acid Phosphatase (ACPP) Function and Localization

• Prostatic acid phosphatase (ACPP) is a non-specific tyrosine phosphatase also known as 5'-nucleotidase (5'-NT), acid phosphatase 3, and ecto-5'-nucleotidase, encoded by the ACP3 gene with multiple isoforms due to alternative splicing.
• Functions as a phosphatase active at acidic pH (4-6), dephosphorylating alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins; also exhibits lipid phosphatase activity, inactivating lysophosphatidic acid in seminal plasma. Related references: PMID:10506173 PMID:15280042 PMID:20498373
• Acts as a tumor suppressor in prostate cancer by dephosphorylating ERBB2 and deactivating MAPK-mediated signaling; additionally functions as an ecto-5'-nucleotidase in dorsal root ganglion neurons, generating adenosine from AMP for pain suppression. Related references: PMID:20498373
• Forms amyloid beta-sheet fibrils (SEVI) in semen that capture HIV virions and enhance infection; SEVI assembly and HIV enhancement can be inhibited by EGCG, WW61 peptide, and surfen; also enhances XMRV infection. Related references: PMID:18083097 PMID:19451623 PMID:19897482 PMID:21677644 PMID:19403677
• Subcellularly localizes to the cell membrane, lysosome membrane, nucleus, cytosol, and is also secreted.
• Highly expressed in normal prostate glandular and ductal epithelial cells, with lower levels in prostate carcinoma; also detected in bladder, kidney, pancreas, lung, cervix, testis, ovary, and skeletal muscle.
• Post-translationally modified as a glycoprotein with disulfide bonds; multiple isoforms exist due to alternative splicing and proteolytic processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 33–386 of human ACPP, which excludes the signal peptide; the antibody may recognize both full-length and processed forms.
• For western blot (WB) with predicted protein weight of 45 kDa, consider using reducing conditions and validate in cell or tissue lysates with known expression.
• For immunohistochemistry (IHC-P), antigen retrieval protocols may be necessary; testing on prostate tissue sections is recommended given high expression in normal prostate.
• Given cross-reactivity with mouse and rat, validate antibody performance in these species by checking sequence homology and using appropriate controls.
• As a polyclonal antibody, it may detect multiple isoforms or processing variants; consider confirming band specificity with siRNA or blocking peptides.
• For ELISA applications, optimize coating concentrations and secondary detection systems; recombinant ACPP protein can serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop platform for IVD raw-material sourcing, providing validated antibodies, antigen reagents, and custom technical services to accelerate diagnostic development. For Prostatic Acid Phosphatase (ACPP) research, we supply high-quality rabbit polyclonal antibodies suitable for WB, IHC-P, and ELISA, along with bulk ancillary reagents and rare target sourcing capabilities. Whether you need reliable antibody pairs, optimized monoclonals, or tailored research support, CamelBio bridges the gap from concept to clinic with efficient and scalable solutions.