Anti-Prostatic Acid Phosphatase (ACPP) Monoclonal Antibody for WB and ELISA - P15309

Core Product Specifications and Parameters

Biological Background: Prostatic Acid Phosphatase (ACPP) Function and Localization

• Prostatic acid phosphatase (ACPP), also designated as 5'-nucleotidase (5'-NT), acid phosphatase 3, ecto-5'-nucleotidase, and protein tyrosine phosphatase ACP3, is encoded by the ACP3 gene in humans.
• Functions as a non-specific tyrosine phosphatase under acidic conditions (pH 4-6), hydrolyzing alkyl, aryl, and acyl orthophosphate monoesters as well as phosphorylated proteins; also possesses lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Related references: PMID:10506173, PMID:15280042
• Acts as a tumor suppressor in prostate cancer by dephosphorylating ERBB2 and deactivating MAPK-mediated signaling pathways. Related references: PMID:20498373
• Exhibits ecto-5'-nucleotidase activity in dorsal root ganglion neurons, generating adenosine from AMP, which functions as a pain suppressor.
• In the context of microbial infection, ACPP forms SEVI amyloid fibrils in semen that capture HIV virions, enhancing infection; this process is inhibited by EGCG, the peptide WW61, or surfen. Additionally, SEVI fibrils boost XMRV infection. Related references: PMID:18083097, PMID:19451623, PMID:19897482, PMID:21677644, PMID:19403677
• Subcellularly, ACPP is found in secreted form, at the cell membrane, lysosome membrane, nucleus, cytoplasm, and cytosol.
• Tissue expression is highest in prostate glandular and ductal epithelial cells, with additional expression in bladder, kidney, pancreas, lung, cervix, testis, ovary, and a weaker signal in a subset of other tissues. An alternative isoform is widely expressed, including in the sarcolemma of skeletal muscle.
• Annotated with keywords including alternative splicing, amyloid, disulfide bond, glycoprotein, hydrolase, lipid metabolism, membrane, nucleus, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human ACPP. This design may enable recognition of both native and denatured protein, making it suitable for applications like WB and ELISA.
• For Western blotting, consider testing whole cell lysates from human prostate cancer cell lines (e.g., LNCaP, PC-3) or transfected cells. The predicted molecular weight is approximately 45 kDa; however, glycosylation may result in a slight shift.
• ELISA applications may benefit from using recombinant ACPP protein or peptide as capture antigen. The antibody’s cross-reactivity with mouse and rat allows for validation in rodent models, but confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, offering full support from concept to clinic. Alongside this Prostatic Acid Phosphatase (ACPP) monoclonal antibody, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing of rare-target reagents. Whether your focus is on prostate cancer biomarkers, phosphatase activity assays, or HIV infection research, our one-stop platform streamlines your procurement and development workflow.