Anti-Prostaglandin E Synthase 2 (PTGES2) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H7Z7

Core Product Specifications and Parameters

Biological Background: PTGES2 Function and Localization

• Also known as mPGES-2, PGES2, GBF1, and C9orf15; belongs to the prostaglandin E synthase family.
• Catalyzes the isomerization of prostaglandin H2 (PGH2) to prostaglandin E2 (PGE2) in vitro, a key step in prostanoid biosynthesis. Related references:
  PMID:12804604 | PMID:17585783 | PMID:18198127
• The biological function of PTGES2 is under debate; it may form a complex with glutathione (GSH) and heme and catalyze the degradation of PGH2 to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA) in vivo (By similarity). Related references:
  PMID:17585783
• Localizes predominantly to the Golgi apparatus membrane and perinuclear cytoplasm.
• Widely expressed in normal tissues, including heart (apex, septum, atria, ventricles), brain (cerebellum, occipital, frontal, parietal lobes), lymph nodes, skeletal muscle, kidney, and trachea; notably absent in thymus and lung.
• Overexpressed in colorectal cancer, suggesting a role in tumorigenesis.
• Post-translational modifications include phosphorylation; the protein is a transmembrane helix-containing membrane protein.
• Involved in fatty acid and lipid metabolism, as well as prostaglandin biosynthesis and metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans a large region (aa 88–377) of human PTGES2, making this polyclonal antibody suitable for detecting multiple epitopes and potentially various isoforms or cleavage products.
• For Western blot (WB), expect a band near the predicted molecular weight of 42 kDa; consider using appropriate positive controls (e.g., lysates from heart or colorectal cancer cell lines) and validate in the relevant sample system.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization to visualize the Golgi and cytoplasmic distribution; co-staining with organelle markers can help confirm localization.
• In ELISA, the antibody can be used for detection or capture, but optimal dilutions must be determined experimentally.
• Cross-reactivity with mouse and rat enables translational studies across these model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your prostaglandin metabolism and cancer biomarker research. Our team can also assist in sourcing rare-target raw materials, streamlining your development from concept to clinic. Contact us to explore custom and catalog solutions for your diagnostic needs.